Product overview Description Anti-CXCR6 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human CXCR6 Reactivity Human, Mouse Content 0.5 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol CXCR6 Full name chemokine (C-X-C motif) receptor 6 Synonyms BONZO; CD186; STRL33; TYMSTR Swissprot O00574
Target Background Bonzo (also designated STRL33.3) and BOB (brother of Bonzo; also designated GRP15) are seven-transmembrane, G protein-coupled receptors that are closely related to chemokine receptor family members. In conjunction with CD4, several chemokine receptors are known to serve as receptors for HIV-1 entry into cells. Bonzo and BOB are utilized by simian immunodeficiency virus (SIV), HIV-2 and M-tropic HIV-1 for cell entry. Bonzo and BOB are both expressed in lymphoid tissues, and BOB is also expressed in colon.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 50-200 Predicted cell location: Cytoplasm Positive control: Human gastric cancer Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml222171(CXCR6 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml222171(CXCR6 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:39 kDa Positive control:Mouse brain tissue Recommended dilution: 200-1000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Mouse brain tissue Primary antibody: ml222171(CXCR6 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes