- 询价
- gelatins
- JLC222145
- 上海
- 2025年07月09日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-CPSF4 antibody
- 宿主：
Rabbit
- 浓度：
0.4 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-CPSF4 antibody
Cat. No. JLC222145
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CPSF4 rabbit polyclonal antibody
Applications ELISA, WB
Immunogen Fusion protein of human CPSF4
Reactivity Human, Mouse, Rat
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CPSF4
Full name cleavage and polyadenylation specific factor 4, 30kDa
Synonyms NAR; NEB1; CPSF30
Swissprot O95639
Target Background
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Applications
Western blotting
Predicted band size:30 kDa
Positive control:Hela and 293T cells
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Hela cells, 293T cells
Primary antibody: ml222145(CPSF4 Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution:2000-5000
Cat. No. JLC222145
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CPSF4 rabbit polyclonal antibody
Applications ELISA, WB
Immunogen Fusion protein of human CPSF4
Reactivity Human, Mouse, Rat
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CPSF4
Full name cleavage and polyadenylation specific factor 4, 30kDa
Synonyms NAR; NEB1; CPSF30
Swissprot O95639
Target Background
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Applications
Western blotting
Predicted band size:30 kDa
Positive control:Hela and 293T cells
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Hela cells, 293T cells
Primary antibody: ml222145(CPSF4 Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution:2000-5000
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Anti-CPSF4 antibody
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