- 询价
- gelatins
- JLC222122
- 上海
- 2025年07月11日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-CKMT2 antibody
- 宿主：
Rabbit
- 浓度：
0.4 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC222122
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CKMT2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human CKMT2
Reactivity Human, Mouse, Rat
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CKMT2
Full name creatine kinase, mitochondrial 2 (sarcomeric)
Synonyms SMTCK
Swissprot P17540
Target Background
Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human gasrtic cancer
Recommended dilution: 100-300 Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 100-300
The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using ml222122(CKMT2 Antibody) at dilution 1/60. (Original magnification: ×200) The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml222122(CKMT2 Antibody) at dilution 1/60. (Original magnification: ×200)
Western blotting
Predicted band size:48 kDa
Positive control:Jurkat cells
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: Jurkat cells
Primary antibody: ml222122(CKMT2 Antibody) at dilution 1/700
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 2000-5000
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