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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-2080R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-Cecropin/FITC
- 抗体名:
Anti-Cecropin/FITC
- 标记物:
FITC标记
- 宿主:
Hyalophora cecropia
- 适应物种:
Hyalophora cecropia
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-Cecropin/FITC |
| 中文名称 | FITC标记的抗菌肽/天蚕素抗体 |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 植物抗体 |
| 研究领域 | 细菌及病毒 植物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Hyalophora cecropia |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 4kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide from Hyalophora cecropia Cecropin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Cecropins are antimicrobial peptides.They were first isolated from the hemolymph of Hyalophora cecropia, from whence the term cecropin was derived. Cecropins lyse bacterial cell membranes; they also inhibit proline uptake and cause leaky membranes. Cecropins constitute a main part of the cell-free immunity of insects. Cecropins are small proteins of about 31 - 37 amino acid residues active against both Gram-positive and Gram-negative bacteria. Cecropins isolated from insects other than Hyalophora cecropia (Cecropia moth) have been given various names; bactericidin, lepidopteran, sarcotoxin, etc. All of these peptides are structurally related. Cecropin P1, an intestinal antibacterial peptide from Sus scrofa (Pig), also belongs to this family. Cecropin family also consists Cecropin A and Cecropin B. Function: Cecropins have lytic and antibacterial activity against several Gram-positive and Gram-negative bacteria. Subcellular Location: Secreted. Post-translational modifications: A protein with the same sequence as cecropin A, but lacking the carboxyl blocking group, has been isolated and called cecropin C (PubMed:7140755). Similarity: Belongs to the cecropin family. Database links:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Cecropin抗菌肽又称天蚕素,具有抗菌、抗病毒、抗肿瘤等活性,因其水溶性好,耐高温,是一种比较理想的抗生素代用品;尤其是其主要抑菌机制,可破坏细胞膜的稳定性,不易产生耐药性。 抗菌肽一般是小肽,可以被动物完全消化,不存在残留性的问题,所以,抗菌肽无论是作为饲料添加剂,兽药还是人药,都有理论上的研究价值和生产中的实际意义 |
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文献和实验抗大鼠波形蛋白单克隆抗体,l:200稀释度),4℃过夜或37℃孵育30分钟,BS漂洗3次,每次5分钟; (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1:100),37℃避光孵育30分钟,PBS漂洗3次,每次5分钟; (8)Hoechst 333442复染细胞核15~20分钟(不是必需步骤); (9)甘油磷酸缓冲液封片,荧光显微镜下镜观察; (10)结果分析:波形蛋白免疫反应阳性细胞胞质呈现绿色荧光,如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
tissue,human testis tissue,human brain tissue IF:Hela,HepG2 另外有 HRP、FITC、Bintin 标记的多抗对应货号分别为: CSB-PA07179B0Rb、CSB-PA07179C0Rb、CSB-PA07179D0Rb 兔抗人淀粉样肽 beta A4 蛋白单克隆抗体 货号:CSB-MA0019501A0m 识别
中。5. 用无菌 PBS 将上述锥形试管填满(至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下,以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞(BD 产品目录号:555899,10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。)8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中(PBS 含有 5% 正常人血清)。9. 加入抗体:CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
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