- 询价
- gelatins
- JLC221861
- 上海
- 2025年07月12日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-ICAM1 antibody
- 宿主：
Rabbit
- 浓度：
0.6 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC221861
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ICAM1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ICAM1
Reactivity Human
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ICAM1
Full name intercellular adhesion molecule 1
Synonyms BB2; CD54; P3.58
Swissprot P05362
Target Background
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human gasrtic cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using ml221861(ICAM1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221861(ICAM1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:58 kDa
Positive control:Raji and huvec cells
Recommended dilution: 1000-5000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Raji cells, huvec cells
Primary antibody: ml221861(ICAM1 Antibody) at dilution 1/1150
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 second
ELISA
Recommended dilution: 2000-10000
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