Product overview Description Anti-YARS2 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human YARS2 Reactivity Human, Mouse, Rat Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol YARS2 Full name tyrosyl-tRNA synthetase 2, mitochondrial Synonyms TYRRS; CGI-04; MLASA2; MT-TYRRS Swissprot Q9Y2Z4
Target Background This gene encodes a mitochondrial protein that catalyzes the attachment of tyrosine to tRNA(Tyr). Mutations in this gene are associated with myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2).
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human gasrtic cancer Recommended dilution: 25-100 Predicted cell location: Cytoplasm Positive control: Human thyroid cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using ml221823(YARS2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221823(YARS2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:53 kDa Positive control:NIH/3T3, A172, HepG2, K562 and Hela cells Recommended dilution: 500-2000
Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-5: NIH/3T3 cells, A172 cells, HepG2 cells, K562 cells, Hela cells Primary antibody: ml221823(YARS2 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds