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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-NIF3L1 antibody
- 宿主:
Rabbit
- 浓度:
0.05 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-NIF3L1 antibody
Cat. No. JLC221595
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-NIF3L1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human NIF3L1
Reactivity Human, Mouse, Rat
Content 0.05 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NIF3L1
Full name NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
Synonyms CALS-7; MDS015; ALS2CR1
Swissprot Q9GZT8
Target Background
NIF3-like protein 1 is a protein that in humans is encoded by the NIF3L1 gene. NIF3L1 has been shown to interact with COPS2. Expression analysis on cell lines revealed strong overexpression in a spermatogonia-derived cell line and a teratocarcinoma cell line. A human NIF3L1/GFP fusion protein showed localization of the protein in the cytoplasm.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221595(NIF3L1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:42 kDa
Positive control:Jurkat cells and human fetal kidney tissue
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Jurkat cells, human fetal kidney tissue
Primary antibody: ml221595(NIF3L1 Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 2000-5000
Cat. No. JLC221595
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-NIF3L1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human NIF3L1
Reactivity Human, Mouse, Rat
Content 0.05 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NIF3L1
Full name NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
Synonyms CALS-7; MDS015; ALS2CR1
Swissprot Q9GZT8
Target Background
NIF3-like protein 1 is a protein that in humans is encoded by the NIF3L1 gene. NIF3L1 has been shown to interact with COPS2. Expression analysis on cell lines revealed strong overexpression in a spermatogonia-derived cell line and a teratocarcinoma cell line. A human NIF3L1/GFP fusion protein showed localization of the protein in the cytoplasm.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221595(NIF3L1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:42 kDa
Positive control:Jurkat cells and human fetal kidney tissue
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Jurkat cells, human fetal kidney tissue
Primary antibody: ml221595(NIF3L1 Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 2000-5000
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Anti-NIF3L1 antibody
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