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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-FABP2 antibody
- 宿主:
Rabbit
- 浓度:
0.6 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Cat. No. JLC220621
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-FABP2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human FABP2
Reactivity Human, Mouse, Rat
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol FABP2
Full name fatty acid binding protein 2, intestinal
Synonyms FABPI; I-FABP
Swissprot P12104
Target Background
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml220621(FABP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:15 kDa
Positive control:Mouse small intestine tissue
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane: Mouse small intestine tissue
Primary antibody: ml220621(FABP2 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 1000-2000
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