- 询价
- 禾午生物
- 国产/进口
- S21282
- 2025年07月08日
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- 库存：
99
- 供应商：
上海禾午生物科技有限公司
- CAS号：
916649-91-7
- 规格：
分析标准品,HPLC≥95%
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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
. Prepare fresh, since crude saponin powder batches are generally fungi infected. Intracellular transport inhibitor Brefeldin A ( Sigma Catalog # B7651) Microscope slides Adhesion slides ( BioRad Catalog # 12550-15) (smeared cells); TC
Immunohistochemisty-Fluorescence Protocol
, store at 4°C in the dark; prepare working dilution just prior to fixation Wash Buffer Earls Buffered Salt Solution (EBSS) (Gibco BRL) Wash Buffer-Saponin EBSS with 0.1 (w/v) Saponin (Sigma Catalog # S4521) and adjust pH to 7.2-7.4 with NaOH
Whole mount antibody staining of zebrafish embryos for markers of segmentation
, 10 minutes each. 3. Incubate overnight at 4oC (cold room) in 0.5 ml primary antibody in 0.2% saponin in PBS. Primary antibodies: (A) znp-1 @ 1/2000 (primary motoneurons) (B)F6@ 1/500 (somite boundaries) (C) no antibody (Control)
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