Rat EGF ELISA Kit (Sandwich EL

ISA) - LS-F24934

价格$690

品牌LSBio
检测范围7.8 - 1000 pg/ml
货号LS-F24934
产地美国
更新日期2025年07月10日

上海康朗生物科技有限公司

12 年钻石会员

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Product Description

LS-F24934 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat EGF in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of EGF as low as 7.8 picograms per milliliter.
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Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

EGF
Synonyms

EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Reactivity

Rat
Specificity

For the detection and quantification of endogenous levels of natural and/or recombinant Rat EGF proteins.
Manual

Intended Sample Types

Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

7.8 - 1000 pg/ml
Sensitivity

7.8 pg/ml
Storage

Short term: 4°C; Long term: see manual.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

Coated 96-well Strip Plate
Standard (Lyophilized)
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
Sample Diluent
Biotinylated Detection Antibody Diluent
HRP-Streptavidin Conjugate Diluent
Wash Buffer (20x)
TMB Substrate
Stop Solution
Adhesive Plate Sealers

Background

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
About EGF

EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Uniprot: P01133 NCBI: NM_001963 NP_001954.2

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