Rat EGF ELISA Kit (Sandwich EL

Rat EGF ELISA Kit (Sandwich ELISA) - LS-F309

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Size
Price
LS-F309-1
1 plate
$800 USD
Available Discounts
10% off 2-4 units
20% off 5+ units
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Product Description

LS-F309 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat EGF in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of EGF as low as 0.819 picograms per milliliter.
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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
EGF
Synonyms
 
EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Reactivity
 
Rat
Specificity
 
The antibody pair provided in this kit recognizes rat EGF.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
 
4-40 fold
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.819 - 80 pg/ml
Sensitivity
 
0.819 pg/ml
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Wash Buffer Concentrate (20X)
• Standard (Lyophilized)
• Biotinylated Detection Antibody
• HRP-Streptavidin Conjugate (200x)
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent A
• Assay Diluent B (5x)
• Adhesive Plate Sealers

Protocol
 

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About EGF
 
EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Uniprot: P01133 NCBI: NM_001963 NP_001954.2

 

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