- ¥2980
- LMAI Bio
- LM-5405R-FITC
- 中国/美国/欧洲
- 2025年07月05日
- IF=1:50-200
- Human, Rat,
- Human, Rat,
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-5405R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-phospho-CK18(Ser60)/FITC
- 抗体名：
Anti-phospho-CK18(Ser60)/FITC
- 标记物：
FITC标记
- 宿主：
Human, Rat,
- 适应物种：
Human, Rat,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-phospho-CK18(Ser60)/FITC |
| 中文名称 | FITC标记的磷酸化细胞角蛋白18抗体 |
| 别 名 | Cytokeratin 18 (phospho S60); p-Cytokeratin 18 (phospho S60); Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; K 18; K18; K1C18_HUMAN; Keratin 18; Keratin D; Keratin type I cytoskeletal 18; keratin, type I cytoskeletal 18; Keratin-18; Keratin18; Kerd; KRT18. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 48kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CK18 around the phosphorylation site of Ser60 [MG(p-S)GG] |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. Function: When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Subunit: Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Subcellular Location: Cytoplasm, perinuclear region. Nucleus, nucleolus. Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. Post-translational modifications: Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue. DISEASE: Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. Similarity: Belongs to the intermediate filament family. Database links: Entrez Gene: 506480 Cow Entrez Gene: 3875 Human Entrez Gene: 16668 Mouse Entrez Gene: 294853 Rat Omim: 148070 Human SwissProt: P05783 Human SwissProt: P05784 Mouse SwissProt: Q5BJY9 Rat Unigene: 406013 Human Unigene: 22479 Mouse Unigene: 103924 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 结构蛋白（Structural Proteins）常用于肿瘤细胞的分化、增殖及转移方面的研究 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验指标：确认流式细胞仪能检测多少个通道，流式抗体每个通道只能选择1种荧光素。各个通道之间的荧光素可以随意搭配。如实验者同时检测三个指标，可以在下图中绿色、黄色和红色三个通道中各选一个适当的荧光素标记，FITC、PE和PE-cy5。切忌所有指标选择同一个通道的荧光标记，以防止荧光的重叠和相互干扰，影响最后结果。因此，流式细胞仪的通道越多，同一份样本能同时做的表面/胞内标志就越多。常用荧光标记包括FITC, PE, PEcy5, PEcy5.5, APC等。 4、同型对照的选择： 流式细胞
FITC, FL2通道检测PE,则需将仪器的补偿设置为FL1-%FL2=1.10，FL2-%FL1=16.52.可以简单理解为：PE荧光染料的1.10%漏到FL1检测通道,需从FL1中减除，而FITC荧光染料的16.52%漏到FL2检测通道，需从FL2中减除。 常见荧光染料亮度比较 荧光染料的亮度可以用来比较不同荧光染料的荧光标记效果，通过阴性细胞群与阳性细胞群的荧光信号之间的关系来表示。但是阴性细胞群的荧光信号受到信号强度、自发荧光、非特异性染色、仪器的电子噪声等多个因素
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
技术资料暂无技术资料 索取技术资料
