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- 详细信息
- 文献和实验
- 技术资料
- 现货状态:
现货
- 供应商:
BIO-RAD
- 规格:
TLS0851
Use Bio-Rad low-profile 0.2 ml 8-tube strips for PCR with volumes of 5–125 µl (200 µl max).
- Low overall height (15.48 mm) reduces condensation
- Ideal for fast PCR, low-volume reactions, and real-time PCR detection systems
- Free of DNase, RNase, and human DNA
- White tubes; also available in clear (TLS-0801)
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, gelatin or BSA to 100ug/ml, and/or non-ionic detergents such as Tween-20 or Nonidet P-40 or Triton X-100 (0.05 - 0.10% v/v) (Innis and Gelfand, 1990)。 Modern formulations may differ considerably, however - they are also generally proprietary. PCR
(BD Biosciences / Clontech). SYBR Green PCR master mix, 200 reactions (Applied Biosystems). Optical tube and cap strips (Applied Biosystems). SuperScript
and template into a 0.5 ml tube, then adding two drops of mineral oil from a blue tip, and adding on top of the oil 38.5 µl of a premix containing all the other components. In this way, it is easy to set up many different primer/template combinations
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