- 询价
- BIO-RAD
- 1708890
- 美国
- 2025年07月22日
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现货
- 供应商：
BIO-RAD
- 规格：
1708890
Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.
Key Features and Benefits
- Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Fast 26 min cDNA synthesis protocol
- Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, µl | Catalog Number |
| 25 | 100 (1 x 100 µl) | 1708890 |
| 100 | 400 (1 x 400 µl) | 1708891 |
| 500 | 2,000 (5 x 400 µl) | 1708891BUN |
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文献和实验of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
有SMART cDNA 合成和文库构建到? TriplEx2 或者Creator 供体载体 pDNR-LIB所需的试剂。因为该试剂盒是特别针对文库构建来设计的，所以它与Clontech™ PCR-Select™cDNA 差减杂交试剂盒不能配合使用。 SMART PCR cDNA Synthesis Kit 可以用来与Clontech™ PCR-Select™ cDNA 差减技术一起使用。总RNA 不能直接用来做差减实验，但可以通过SMART PCR cDNA Synthesis Kit 来扩增出
Reverse Transcription of RNA- 20l rxn
. This is the quivalent of ~ 25-50 ug total RNA. (X-H uses much less). I have seen consistently better results with oligo dT primed RNA even for very long RNA where 5 prime regions are to be amplified. Many people swear by superscript in our experience biolabs
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