Hard-Shell® 96-Well PCR Plates

Hard-Shell 96-Well Semi-Skirted PCR Plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The full-height (20.75 mm) wells fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers.

Key Features and Benefits

• Reaction volumes of 5–125 µl (350 µl maximum)
• Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
• Warp-free half-height skirt for improved robotic handling and labeling surface
• Black lettering for easy well identification
• Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA

Options

• White-well option for increased fluorescent signal strength
• Color-coded skirt option with clear wells
• Low-cost, user-readable bar code option for convenient database tracking

Plate Dimensions

Microplate Dimensions	Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates (HSS-9xxx)
Length at top plane	127.76 mm
Width at top plane	85.47 mm
Height overall	20.75 mm
Well depth	19.85 mm
Well diameter at opening	5.50 mm
Well diameter at bottom of conical section	2.69 mm
Well volume	350 μl
Well spacing	9.00 mm
Well angle	17.5°
Well offset
Left edge to well A1	14.37 mm
Top edge to well A1	11.23 mm
Left edge to H12	113.37 mm
Top edge to H12	74.23 mm

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.