- ¥2980
- LMAI Bio
- LM-5581R-FITC
- 中国/美国/欧洲
- 2025年07月08日
- IF=1:50-200
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-5581R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-phospho-PIK3C3(Ser164)/FITC
- 抗体名：
Anti-phospho-PIK3C3(Ser164)/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- 适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-phospho-PIK3C3(Ser164)/FITC |
| 中文名称 | FITC标记的磷酸化磷脂酰肌醇激酶3催化亚单位3抗体 |
| 别 名 | PIK3C3(phospho S164); p-PIK3C3(phospho S164); p-PIK3C3(Ser164); PI 3 Kinase Class 3; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphoinositide 3 kinase class 3; PI3 kinase type 3; PI3K type 3; PIK3C3; PtdIns 3 kinase type 3; Vps 34; Vps34; hVps34; MGC61518. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 98kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PIK3C3 around the phosphorylation site of Ser164 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: PI 3 Kinase Class 3 is a member of the PI3/PI4-kinase family. It is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes. It is ubiquitously expressed, with a highest expression in skeletal muscle. Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Subunit: Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3R4. Subcellular Location: Midbody. Late endosome. Tissue Specificity: Ubiquitously expressed, with a highest expression in skeletal muscle. Similarity: Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain. Database links: Entrez Gene: 5289 Human Entrez Gene: 225326 Mouse Entrez Gene: 65052 Rat Omim: 602609 Human SwissProt: Q8NEB9 Human SwissProt: Q6PF93 Mouse SwissProt: O88763 Rat Unigene: 464971 Human Unigene: 194127 Mouse Unigene: 30010 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验以及饱和湿度条件下松盖培养，每 2~3 d 换液一次，当细胞长至 80% 铺满时再消化传代。实验分组H1299 细胞-Con 组lv-shCON 转染的 H1299 细胞lv-shIFITM3 转染的 H1299 细胞Annexin V-FITC/PI 检测用去离子水将 10×Binding Buffer 稀释成 1×Binding Buffer；细胞收集：悬浮细胞收集：离心 5 分钟；贴壁细胞：用不含 EDTA 的胰酶消化收集后（注：胰酶消化的时间不宜过长，否则会影响细胞膜上磷脂酰丝氨酸
在胞质中以单体形式存在，发出绿色的荧光。可以在荧光显微镜下，或流式检测。采用488nm激发，其检测波长分别是527nm和590nm。整个实验过程操作简单，只需要30min就可以见到结果。Caspase家族可以检测的分子非常多，也有不少商业的试剂盒可以应用。即使没有相应的试剂盒，只要有相应抗体基本上是可以检测的，具体的方法是参照细胞内蛋白检测的步骤。在细胞凋亡过程中伴随着一系列的形态特征改变，细胞膜的改变是这些特征中较早出现的一种。在凋亡细胞中，细胞膜磷脂酰丝氨酸（PS）从细胞膜的内侧翻转到细胞
自己知识产权的人类新基因,前期的功能研究表明,它是促进细胞凋亡的增强剂.利用荧光 素(FITC)标记的TFAR19单克隆抗体为探针,对细胞凋亡过程中TFAR19蛋白的表达水平及定位研究发现,凋亡早期TFAR19表达水平增高并出 现快速核转位现象...查看原文 了解更多有用的、有意思的前沿资讯以及酷炫的实验方法,可以向大师兄申请微信好友 师兄微信号:shixiongcoming
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