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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Dog
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
To be determined
- 供应商:
康朗生物
- 规格:
96T
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Product Description
LS-F14748 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Dog Dog IgM. It is based upon a Custom assay principle.More Dog IgM Products
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Specifications
TypeCustom ELISA kit
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Target
Dog IgM
Reactivity
Dog
Manual
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
To be determined
Sensitivity
To be determined
Storage
Short term: 4°C; Long term: see manual.
Kit Components
Typical components include:
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Wash Buffer (25x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About Dog IgM
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen. The spleen where plasmablasts reside is the major site of specific IgM production. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer.
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
]=2.5 单抗HRPO标记是否成功? 直观验证: 七、HSV1单克隆抗体的应用 血清中IgM抗体的测定: 血清中IgM抗体的检测用于传染病的早期诊断中。 间接法ELISA一般仅适用于检测总抗体或IgG抗体。 血清中针对某抗原的IgM和IgG同时存在,大量存在的IgG会干扰IgM抗体的测定。 间接ELISA测定IgM抗体,必须先将标本用A蛋白处理或抗IgG抗体中和,以除去IgG的干扰。 捕获法ELISA: 在临床检验中测定
摘要 目的 探讨循环免疫复合物与脑梗死的关系。方法 采用酶联免疫吸附试验(ELISA)方法测定脑梗死患者血中巨细胞病毒抗体(CMV-IgG与CMV-IgM)及衣原体抗体(CP-IgG),并与健康对照组比较。结果 脑梗死组循环免疫复合物显著升高(P﹤0.01),其中CMV-IgM在发病早期明显升高(P﹤0.01),随着病情康复,其水平逐渐下降。CMV-IgG和CP-IgG也呈现类似变化。结论 脑梗死患者血中CMV-IgG、CMV-IgM和CD-IgG升高,循环复合物对脑梗死的发生有一定的不良
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