人波形蛋白(Vimentin)

纯度

> 95 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

Testing in progress

蛋白构建

A DNA sequence encoding the human VIM (Met 1-Glu466) (P08670) was expressed, with a C-terminal polyhistidine tag.

NCBI Reference No.

P08670

表达宿主

Baculovirus-Insect Cells

种属

Human

预测 N 端

Met

分子量

The secreted recombinant human VIM consists of 476 amino acids and predicts a molecular mass of 55 KDa. The apparent molecular mass of the protein is approximately 56 KDa in SDS-PAGE under reducing conditions due to glycosylation.

缓冲液

Lyophilized from sterile 40% acetonitrile, 0.1% TFA
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.