1-甲基咪唑 Green Alternative

Green Fluorescent Protein as a Tracer in Chimeric Tissues: The Power of Vapor Fixation

Green fluorescent protein (GFP) and its variants, small, highly soluble proteins, are routinely used as reporters for patterns of gene expression and the origin of cells in transplantation experiments. When not linked as fusion proteins

Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Construct

Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain

UV Shadowing

of nonisotopic probes synthesized with Ambion's MAXIscript™ and BrightStar™ Psoralen-Biotin Kits. The alternative to UV shadowing is staining with ethidium bromide or acridine orange and requires subsequent extraction of the dye. The detection limit of UV