Acetyl coenzyme A is suitable in CAT reporter assay for the measurement of chloramphenicol acetyltransferase by incubating the cell extract with 0.1 μCi [14C] chloramphenicol and 0.5mM acetyl-CoA.[1][2][3][4]
Biochem/physiol Actions
在酶催化乙酰基转移反应中,乙酰辅酶 A 是必要的辅因子和酰基载体。它可以通过线粒体中酸的氧化脱羧反应,或者长链脂肪酸的氧化反应,或者某些氨基酸的氧化降解反应而形成。它是脂质生物合成的重要前体以及所有脂肪酸的碳源。它是酸羧化酶的正调节物。它是神经递质乙酰胆碱的前体。
Alterations in the gene encoding acetyl-CoA have been linked to Alzheimer′s disease and mild cognitive impairment.[5] Defects in the gene cause myasthenic syndrome associated with episodic apnea. [6][7]
Other Notes
For more more technical information and a complete list of Coenzyme A deriviatives visit the Acyl Transfer Reagents Resource.
包装
1, 5 mg in glass bottle
10, 25, 100 mg in poly bottle
Preparation Note
酶法制备
Reconstitution
Acetyl CoA is soluble in deionized water at 100 mg/mL. Acetyl CoA is stable in neutral and moderately acidic solutions but will hydrolyze in alkaline and strongly acidic solutions. Aqueous solutions stored in single-use aliquots are stable for up to two weeks at −20°C and up to 6 months at −80°C.
Physical properties
This moisture-sensitive powder should be stored desiccated at −20°C.