- ￥474.44
- SIGMA
- P0899
- 美国
- 2026年01月18日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Poly-D-lysine hydrobromide
- CAS号：
27964-99-4
- 保质期：
2年
- 供应商：
上海瓦兰生物
- 保存条件：
-20°
- 规格：
10MG
性质
| Related Categories | Amino Acids, Homopolymers, Polyamino Acids,Polylysine and Polyornithine Attachment Factors, 生化试剂 更多... |
| InChI Key | MEXAGTSTSPYCEP-UHFFFAOYSA |
| mol wt | mol wt 70,000-150,000 |
| storage temp. | −20°C |
产品描述
Components
分子量 >30,000 的聚赖氨酸适用于促进细胞粘附到固体基质。
Analysis Note
This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
Application
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Poly-D-lysine hydrobromide is used for the following applications:
• Used in animal cell culture[7]
• Rat cortical neuron cultures[5]
• Cell culture and siRNA treatment[1]
• Primary cell cultures and adult neuronal network model[4]
• Cortical astrocyte culture[3]
• Immunofluorescence staining (HDF cells were plated onto poly-D lysine (P0899, Sigma-Aldrich) coated glass coverslips)[6]
• NPC collection and culture conditions[2]
Biochem/physiol Actions
This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.
Caution
无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在
包装
1 g in poly bottle
50, 100, 500 mg in poly bottle
10 mg in glass bottle
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验POLY-L-LYSINE COATED SLIDES SOLUTIONS --5% Chromic acid --0.01% Poly-L-Lysine (PLL) - made from a dry protein, with distilled water. (100mg/1 litre) This procedure can be carried out on either a ski or carousel staining
Silane and Poly-L-Lysine Coating of Microscope Slides
free environment for 5 minutes. Poly-L-Lysine Coating a) Smear 20ul of poly-l-lysine over each slide. b) Air dry in a dust free environment. Silated Slides a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma
Research and development related to nonviral gene carriers comprising chemically synthesized molecules has increased enormously during the past decade. Polycationic polymers and cationic lipids have constituted the main themes of the studies
技术资料