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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Snail蛋白抗体使用说明书
- 抗体英文名:
Anti-Snail/SNAI 1
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海博湖
- 库存:
大量
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human Anail C-terminus
- 规格:
0.2ml/200μg
英文名称 Anti-Snail/SNAI 1
中文名称 Snail蛋白抗体使用说明书
别 名 dJ710H13.1; Protein sna; Protein snail homolog; SLUGH2; SNA; Sna protein; SNAH; SNAI1; Snail homolog 1 (Drosophila); Zinc finger protein SNAI1; SNAI1_HUMAN; Protein snail homolog 1; dJ710H13.1; SNAIL; SNAIL1; Protein sna.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 肿瘤 心血管 细胞生物 信号转导 转录调节因子 锌指蛋白 表观遗传学
蛋白分子量 predicted molecular weight: 29kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Anail C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
Snail蛋白抗体使用说明书产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The zinc finger transcription factor 'SNAIL' was first identified in Drosophila and, along with 'twist', a basic helix-loop-helix transcription factor, is indispensable for mesoderm formation. SNAIL is a repressor of mouse E-cadherin transcription, with expression of SNAIL inversely correlated with expression of E-cadherin. Abnormal expression of SNAIL could underlie the tumorigenic conversion of epithelia associated with loss of E-cadherin expression through screening mouse and human cell lines and by in situ hybridization of primary human tumors undergoing malignant progression.
Function : Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.
Subunit : Interacts with FBXL14 and GSK3B. Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif. Interacts (via SNAG domain) with WTIP (via LIM domains). Interacts (via SNAG domain) with LIMD1 (via LIM domains), and AJUBA (via LIM domains). Interacts with LOXL2 and LOXL3.
Subcellular Location : Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
Tissue Specificity : Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
Post-translational modifications : Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
Similarity : Belongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Database links :
UniProtKB/Swiss-Prot: O95863.2
Entrez Gene: 6615 Human
Omim: 604238 Human
SwissProt: O95863 Human
Unigene: 48029 Human
Snail蛋白主要用于消化系统肿瘤方面的研究
Snail蛋白抗体使用说明书具有全、新、优、品、好四大特点:
全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
新:产品更新速度较快,基本上每周都有新产品出现。
优:产品质量好,投诉比较少。
好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
我们提供的标记服务,可根据您科研的需求,满足您的需要,质量保证,价格合理。
一、标记流程
委托标记服务,请下载并详细填写《委托标记申请单》,待我公司标记室技术人员研究、确认后答复,并办理委托标记事宜。
二、各种标记物制备的收费标准(RMB)
我们不但提供抗体和大分子蛋白的标记服务,还提供小分子多肽及小分子蛋白的各种标记服务,提供标记化合物的服务(此化合物必须适用于标记),价格另议。
一抗和二坑的区别:
第一抗体就是平常所说的抗体,即能和抗原特异性结合。
第二抗体是能和抗体结合的,即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体,二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说,抗原进入机体刺激机体免疫系统产生免疫应答,由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团,而且一抗可以至少结合两种其他基团(底物和二抗)。
一抗:可以特异结合底物,就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗:可以和一抗结合,并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),则不需要二抗。但这样成本很高,因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记,再来检测一抗。而一抗识别底物。这样,当一抗结合到底物上,就可以通过二抗检测出来。
E.coli O6 (Escherichia coli O6) E.coli O6大肠杆菌菌体蛋白Multi-class antibodies规格: 0.5mg
吸引素抗体 Anti-ATRN 0.1ml
Rhesus antibody Rh Phospho-EGFR (Ty72) 化表皮生长因子受体抗体 规格 0.1ml
M199培养液 250ml 国产
ZNF323 英文名称: 指蛋白323抗体 0.1ml
DPPA2 英文名称: 多能发育相关基因2抗体 0.1ml
吸引素抗体 Anti-ATRN 0.1ml
ERK(Extracellular signal-regulated kinase) 原活化蛋白激酶抗原Multi-class antibodies规格: 0.5mg
精酸加压素受体2抗体 Anti-AVPR2 0.1ml
Rhesus antibody Rh Phospho-EGFR (Ty01) 化表皮生长因子受体抗体 规格 0.1ml
16培养液 250ml 国产
ZNT8 英文名称: 转运体8蛋白抗体 0.2ml
DTNB 英文名称: 肌Dystrobrevin β蛋白抗体 0.2ml
精酸加压素受体2抗体 Anti-AVPR2 0.1ml
ZNF93 英文名称: 指蛋白93抗体 0.2ml
DNAH9 英文名称: 轴丝动力蛋白重链9抗体 0.2ml
β-抑制蛋白2抗体 Anti-β-arrestin 2 0.1ml
Anti-TRF/Gold 金离子标记抗转铁蛋白抗体IgGMulti-class antibodies规格: 0.5ml(15nm)
Rhesus antibody Rh phospho-eIF4EBP1(Thr36) 化4E结合蛋白1抗体 规格 0.1ml
rhEGF(rh-Epidermal growth factor) 重组人表皮生长因子Multi-class antibodies规格: 100ug
RILPL2 is a 211 amino acid protein that belongs to the RILPL family. RILPL2 does not regulate lysosomal morphology or distribution. RILPL2 shares 32% and 18% amino acid identity with RILPL1 and RILP, respectively. RILPL2 as a novel interacting partner for the actin-based molecular motor MyoVa, and has a novel role for RILPL2 in controlling neuronal morphogenesis. It has been suggested that there is also a novel role for RILPL2 in the regulation of cellular shape and dendritic-spine morphogenesis, probably via the Rac1-Pak pathway. PCR analysis of human tissues detects highest RILPL2 expression in lung, followed by placenta. Lower expression is detected in liver, kidney, pancreas, heart and brain, but no expression is detected in skeletal muscle. The RILPL2 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish, and maps to human chromosome 12q24.31.
Function : Involved in cell shape and neuronal morphogenesis, positively regulating the establishment and maintenance of dendritic spines. May activate RAC1
Subunit : Interacts (via N-terminus) with MYO5A, the interaction is required for its role in dendrite formation. Interacts with RAC1 (By similarity).
Subcellular Location : Cytoplasm
Tissue Specificity : Widely expressed. Expressed at higher level in lung.
Similarity : Belongs to the RILPL family.
Contains 1 RILP-like domain.
Database links : UniProtKB/Swiss-Prot: Q969X0.1
Anti-HSP-90 Beta /HRP 辣根过氧化物酶标记热休克蛋白90β抗体IgGMulti-class antibodies规格: 0.2ml
pig IgM (亲和纯化) 猪IgMMulti-class antibodies规格: 1mg
人类白细胞抗原G/组织相容性白细胞抗原G抗体 Anti-HLA-G 0.1ml
Rabbit Anti-Biotin 兔抗生物素抗体IgG 0.2ml
F1 protein (YERSINIA PESTIS) 英文名称: 鼠疫F1蛋白/鼠疫耶尔森氏菌荚膜抗原F1单克隆抗体 0.1ml
Rhesus antibody Rh phospho-RGS19(Ser151) 化G蛋白信号转导调节因子19抗体 规格 0.1ml
pig IgM (亲和纯化) 猪IgMMulti-class antibodies规格: 1mg
Snail蛋白抗体使用说明书NSE (neuron specific enolase) 神经元特异性烯醇化酶抗原Multi-class antibodies规格: 0.5mg
Anti-phospho-arfaptin 2(Ser260) 化ADP核糖基化因子结合蛋白2抗体Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh phospho-ATF2(Ser322) 化活化复制因子2抗体 规格 0.1ml
Tr K C 浓缩液 0.1ml 进口分装
Ulex Europaeus Lectin 1 英文名称: 荆豆凝集素1抗体 0.1ml
DNA polymerase subunit II 英文名称: DNA聚合酶α2抗体 0.2ml
Anti-phospho-arfaptin 2(Ser260) 化ADP核糖基化因子结合蛋白2抗体Multi-class antibodies规格: 0.1ml
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文献和实验sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗,这种抗体有单抗的吧? 用于FCM: 1:20-100,这是说用于流式的稀释倍数,是不是稀释倍数越小越好呢? 同型对照抗体是不是必须要做的?如何选择阴型同型对照抗体呢! 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助! freecell 抗体的稀释倍数是需要优化
4H5N.HI+C5H5N.HSO4CH3根据滴定反应中所消耗的卡尔-费休试剂量来算出样品中水份的含量。二、仪器性能及适应范围:1、仪器性能: a、测量范围:30×10-6~100%。 b、以水为标样,测定卡尔-费休试剂的水当量,平行测定相对误差≤3%。 c、电源电压:交流220±10%。2、适应范围:本仪器主要用于测定化肥、医药、食品、轻工、化工原料以及其它工业产品中的水份含量。根据资料及美国材料协会标准ASTM,使用卡尔-费休法可直接测定的化合物包括: 有机化合物-饱和的不饱和的碳氢
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人氧化低密度脂蛋白抗体 (OLab)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 OLab 单抗包被于酶标板上,标准品和样品中的 OLab与单抗结合,加入生物素化的抗人 OLab
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