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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
肌肉骨骼受体酪氨酸激酶抗体
- 抗体英文名：
Anti-MUSK
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:50-200
- 宿主：
详见说明书
- 供应商：
上海谷研
- 库存：
53
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human MUSK
- 规格：
KLH conjugated synthetic peptide derived from human MUSK
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg抗体的多样性：
抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。
英文名称 Anti-MUSK
中文名称 肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg
别 名 skeletal receptor tyrosine-protein kinase; MDK 4; MDK4; Muscle; Muscle skeletal receptor tyrosine kinase; Muscle skeletal receptor tyrosine protein kinase; Muscle specific kinase receptor; Muscle specific tyrosine kinase receptor; Muscle specific tyrosine protein kinase receptor; Muscle-specific kinase receptor; Muscle-specific tyrosine-protein kinase receptor; MuSK; Neural fold somite kinase 1; Neural fold somite kinase 2; Neural fold somite kinase 3; Neural fold somite kinase1; Neural fold somite kinase2; Neural fold somite kinase3; Nsk 1; Nsk 2; Nsk 3; Nsk1; Nsk2; Nsk3; Nsk-1; Nsk-2; Nsk-3; Receptor tyrosine kinase MuSK; Skeletal muscle receptor tyrosine kinase; MUSK_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow
产品类型 一抗
研究领域 细胞生物 神经生物学 信号转导 激酶和磷酸酶 细胞类型标志物 细胞骨架 细胞外基质
蛋白分子量 predicted molecular weight：93kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human MUSK
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg产品应用 WB=1:100-500 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:50-200
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Receptor tyrosine kinases (RTKs) represent an important class of transmembrane signaling molecules. Binding of the extracellular domain of an RTK to its cognate ligand leads to receptor dimerization and the activation of the intrinsic tyrosine kinase activity of its intracellular kinase domain. The specificity of this type of cellular communication is conferred in part by the distribution of the receptor, which determines the cells that are capable of responding to a given ligand. MuSK, for muscle-specific kinase, is an RTK that is uniquely specific to the skeletal muscle lineage. MuSK is expressed at low levels in proliferating myoblasts, but is induced upon terminal differentiation and myotube fusion. In the embryo, MuSK is expressed in developing muscle, but its level of expression is dramatically reduced in mature muscle, where it is abundant only at the neuromuscular junction. The human MuSK gene maps to chromosome 9q31.3, overlapping a region containing the Fukuyama muscular dystrophy mutation.
Function ：
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation
Subunit ：
Monomer (By similarity). Homodimer (Probable). Interacts with LRP4; the heterodimer forms an AGRIN receptor complex that binds AGRIN resulting in activation of MUSK (By similarity). Forms a heterotetramer composed of 2 DOK7 and 2 MUSK molecules which facilitates MUSK trans-autophosphorylation on tyrosine residue and activation. Interacts (via cytoplasmic part) with DOK7 (via IRS-type PTB domain); requires MUSK phosphorylation. Interacts with DVL1 (via DEP domain); the interaction is direct and mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Interacts with PDZRN3; this interaction is enhanced by agrin (By similarity). Interacts with FNTA; the interaction is direct and mediates AGRIN-induced phosphorylation and activation of FNTA (By similarity). Interacts with CSNK2B; mediates regulation by CK2 (By similarity). Interacts (via the cytoplasmic domain) with DNAJA3 (By similarity). Interacts with NSF; may regulate MUSK endocytosis and activity (By similarity). Interacts with CAV3; may regulate MUSK signaling (By similarity). Interacts with RNF31 (By similarity).
Subcellular Location ：
Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein (Probable). Note=Localizes to the postsynaptic cell membrane of the neuromuscular junction
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg实验步骤
① 滴加0.01mol/L，pH7.4的PBS于待检标本片上，10min后弃去，使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液，使其完全覆盖标本，置于有盖搪瓷盒内，保温一定时间（参考：30min）。
③ 取出玻片，置玻片架上，先用0.01mol/L，pH7.4的PBS冲洗后，再按顺序过0.01mol/L，pH7.4的PBS三缸浸泡，每缸3-5 min，不时振荡。
④ 取出玻片，用滤纸吸去多余水分，但不使标本干燥，加一滴缓冲甘油，以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度，一般可用“+”表示：
（-）无荧光；（±）极弱的可疑荧光；（+）荧光较弱，但清楚可见；（++）荧光明亮；（+++ --++++）荧光闪亮。待检标本特异性荧光染色强度达“++”以上，而各种对照显示为（±）或（-），即可判定为阳性。
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg现货供应，货期短，为您提供售前、售中、售后服务，保证实验结果，无效可以免费退换，提供免费代测服务，产品效价高、种类全、价格实惠 ，欢迎来电咨询！
Rabbit anti-MG IgG/Alexa Fluor 488 Alexa Fluor 488标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/Alexa Fluor 555 Alexa Fluor 555标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/Alexa Fluor 647 Alexa Fluor 647标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/PE-Cy5.5 PE-Cy5.5标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/AP 碱性磷酸酶（AP）标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/Gold 胶体金标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/HRP 辣根过氧化物酶标记的兔抗长爪沙鼠IgG 特价促销
Rabbit Anti-Mink IgG/HRP 辣根过氧化物酶标记的兔抗水貂IgG 特价促销
Rabbit Anti-Mink IgG/Cy3 Cy3标记的兔抗水貂IgG 特价促销
Rabbit Anti-Mink IgG/Cy5 Cy5标记的兔抗水貂IgG 特价促销
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μgApoA1 抗體, 兔單抗 现货促销
APOA4 / Apolipoprotein A-IV 抗體, 兔多抗 现货促销
APOA4 / Apolipoprotein A-IV 抗體, 兔多抗, 抗原親和純化 现货促销
ApoE / Apolipoprotein E 抗體, 兔多抗 现货促销
APOE / apolipoprotein E 抗體, 兔多抗, 抗原親和純化 现货促销
Apolipoprotein H / APOH 抗體, 鼠單抗 现货促销
Apolipoprotein H / APOH 抗體, 兔單抗 现货促销
注意事项
1）对荧光标记的抗体的稀释，要保证抗体的蛋白有一定的浓度，一般稀释度不应超过1：20，抗体浓度过低，会导致产生的荧光过弱，影响结果的观察。
2）染色的温度和时间需要根据各种不同的标本及抗原而变化，染色时间可以从10 min到数小时，一般30 min已足够。染色温度多采用室温（25℃左右），高于37℃可加强染色效果，但对不耐热的抗原（如流行性乙型脑炎病毒）可采用0-2℃的低温，延长染色时间。低温染色较37℃30 min效果好的多。
3）为了保证荧光染色的正确性，首次试验时需设置下述对照，以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照：标本加1-2滴0.01mol/L，pH7.4的PBS。
② 特异性对照（抑制试验）：标本加未标记的特异性抗体，再加荧光标记的特异性抗体。
③ 阳性对照：已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光，阳性对照和待检标本呈强荧光，则为特异性阳性染色。
4）一般标本在高压汞灯下照射超过3min，就有荧光减弱现象，经荧光染色的标本zui好在当天观察，随着时间的延长。
抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。
英文名称 Anti-MUSK
中文名称 肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg
别 名 skeletal receptor tyrosine-protein kinase; MDK 4; MDK4; Muscle; Muscle skeletal receptor tyrosine kinase; Muscle skeletal receptor tyrosine protein kinase; Muscle specific kinase receptor; Muscle specific tyrosine kinase receptor; Muscle specific tyrosine protein kinase receptor; Muscle-specific kinase receptor; Muscle-specific tyrosine-protein kinase receptor; MuSK; Neural fold somite kinase 1; Neural fold somite kinase 2; Neural fold somite kinase 3; Neural fold somite kinase1; Neural fold somite kinase2; Neural fold somite kinase3; Nsk 1; Nsk 2; Nsk 3; Nsk1; Nsk2; Nsk3; Nsk-1; Nsk-2; Nsk-3; Receptor tyrosine kinase MuSK; Skeletal muscle receptor tyrosine kinase; MUSK_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow
产品类型 一抗
研究领域 细胞生物 神经生物学 信号转导 激酶和磷酸酶 细胞类型标志物 细胞骨架 细胞外基质
蛋白分子量 predicted molecular weight：93kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human MUSK
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg产品应用 WB=1:100-500 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:50-200
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Receptor tyrosine kinases (RTKs) represent an important class of transmembrane signaling molecules. Binding of the extracellular domain of an RTK to its cognate ligand leads to receptor dimerization and the activation of the intrinsic tyrosine kinase activity of its intracellular kinase domain. The specificity of this type of cellular communication is conferred in part by the distribution of the receptor, which determines the cells that are capable of responding to a given ligand. MuSK, for muscle-specific kinase, is an RTK that is uniquely specific to the skeletal muscle lineage. MuSK is expressed at low levels in proliferating myoblasts, but is induced upon terminal differentiation and myotube fusion. In the embryo, MuSK is expressed in developing muscle, but its level of expression is dramatically reduced in mature muscle, where it is abundant only at the neuromuscular junction. The human MuSK gene maps to chromosome 9q31.3, overlapping a region containing the Fukuyama muscular dystrophy mutation.
Function ：
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation
Subunit ：
Monomer (By similarity). Homodimer (Probable). Interacts with LRP4; the heterodimer forms an AGRIN receptor complex that binds AGRIN resulting in activation of MUSK (By similarity). Forms a heterotetramer composed of 2 DOK7 and 2 MUSK molecules which facilitates MUSK trans-autophosphorylation on tyrosine residue and activation. Interacts (via cytoplasmic part) with DOK7 (via IRS-type PTB domain); requires MUSK phosphorylation. Interacts with DVL1 (via DEP domain); the interaction is direct and mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Interacts with PDZRN3; this interaction is enhanced by agrin (By similarity). Interacts with FNTA; the interaction is direct and mediates AGRIN-induced phosphorylation and activation of FNTA (By similarity). Interacts with CSNK2B; mediates regulation by CK2 (By similarity). Interacts (via the cytoplasmic domain) with DNAJA3 (By similarity). Interacts with NSF; may regulate MUSK endocytosis and activity (By similarity). Interacts with CAV3; may regulate MUSK signaling (By similarity). Interacts with RNF31 (By similarity).
Subcellular Location ：
Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein (Probable). Note=Localizes to the postsynaptic cell membrane of the neuromuscular junction
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg实验步骤
① 滴加0.01mol/L，pH7.4的PBS于待检标本片上，10min后弃去，使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液，使其完全覆盖标本，置于有盖搪瓷盒内，保温一定时间（参考：30min）。
③ 取出玻片，置玻片架上，先用0.01mol/L，pH7.4的PBS冲洗后，再按顺序过0.01mol/L，pH7.4的PBS三缸浸泡，每缸3-5 min，不时振荡。
④ 取出玻片，用滤纸吸去多余水分，但不使标本干燥，加一滴缓冲甘油，以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度，一般可用“+”表示：
（-）无荧光；（±）极弱的可疑荧光；（+）荧光较弱，但清楚可见；（++）荧光明亮；（+++ --++++）荧光闪亮。待检标本特异性荧光染色强度达“++”以上，而各种对照显示为（±）或（-），即可判定为阳性。
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg现货供应，货期短，为您提供售前、售中、售后服务，保证实验结果，无效可以免费退换，提供免费代测服务，产品效价高、种类全、价格实惠 ，欢迎来电咨询！
Rabbit anti-MG IgG/Alexa Fluor 488 Alexa Fluor 488标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/Alexa Fluor 555 Alexa Fluor 555标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/Alexa Fluor 647 Alexa Fluor 647标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/PE-Cy5.5 PE-Cy5.5标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/AP 碱性磷酸酶（AP）标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/Gold 胶体金标记的兔抗长爪沙鼠IgG 特价促销
Rabbit anti-MG IgG/HRP 辣根过氧化物酶标记的兔抗长爪沙鼠IgG 特价促销
Rabbit Anti-Mink IgG/HRP 辣根过氧化物酶标记的兔抗水貂IgG 特价促销
Rabbit Anti-Mink IgG/Cy3 Cy3标记的兔抗水貂IgG 特价促销
Rabbit Anti-Mink IgG/Cy5 Cy5标记的兔抗水貂IgG 特价促销
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μgApoA1 抗體, 兔單抗 现货促销
APOA4 / Apolipoprotein A-IV 抗體, 兔多抗 现货促销
APOA4 / Apolipoprotein A-IV 抗體, 兔多抗, 抗原親和純化 现货促销
ApoE / Apolipoprotein E 抗體, 兔多抗 现货促销
APOE / apolipoprotein E 抗體, 兔多抗, 抗原親和純化 现货促销
Apolipoprotein H / APOH 抗體, 鼠單抗 现货促销
Apolipoprotein H / APOH 抗體, 兔單抗 现货促销
注意事项
1）对荧光标记的抗体的稀释，要保证抗体的蛋白有一定的浓度，一般稀释度不应超过1：20，抗体浓度过低，会导致产生的荧光过弱，影响结果的观察。
2）染色的温度和时间需要根据各种不同的标本及抗原而变化，染色时间可以从10 min到数小时，一般30 min已足够。染色温度多采用室温（25℃左右），高于37℃可加强染色效果，但对不耐热的抗原（如流行性乙型脑炎病毒）可采用0-2℃的低温，延长染色时间。低温染色较37℃30 min效果好的多。
3）为了保证荧光染色的正确性，首次试验时需设置下述对照，以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照：标本加1-2滴0.01mol/L，pH7.4的PBS。
② 特异性对照（抑制试验）：标本加未标记的特异性抗体，再加荧光标记的特异性抗体。
③ 阳性对照：已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光，阳性对照和待检标本呈强荧光，则为特异性阳性染色。
4）一般标本在高压汞灯下照射超过3min，就有荧光减弱现象，经荧光染色的标本zui好在当天观察，随着时间的延长。
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技术资料暂无技术资料 索取技术资料
肌肉骨骼受体酪氨酸激酶抗体0.1ml/100μg 0.2ml/200μg
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