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- 20874-1-AP
- 中国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 抗体英文名：
Cadherin, Epithelial (CDHE)
- 保质期：
E-cadherin
- 供应商：
上海研卉生物科技有限公司
- 规格：
100ul
- KD/KO Validated
E-cadherin Polyclonal antibody
E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat No : 20874-1-AP
经过测试的应用
| Positive WB detected in | A431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human breast cancer tissue, mouse colon tissue, human colon tissue, mouse skin tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, human breast cancer tissue, mouse embryo tissue, mouse small intestine tissue, A431 cells |
| Positive FC detected in | HepG2 cells |
Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 1318 publications below |
| IHC | See 250 publications below |
| IF | See 284 publications below |
| FC | See 3 publications below |
| CoIP | See 5 publications below |
产品信息
20874-1-AP targets E-cadherin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | CoIP, FC, IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, pig, canine, bovine |
| Immunogen | E-cadherin fusion protein Ag14973 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
| Synonyms | Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin |
| Calculated molecular weight | 882 aa, 97 kDa |
| Observed molecular weight | 120-125 kDa |
| GenBank accession number | BC141838 |
| Gene symbol | CDH1 |
| Uniprot | P12830 |
| Gene ID (NCBI) | 999 |
| RRID | AB_10697811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
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文献和实验发表文章
| Species | Application | Title |
|---|---|---|
|
Science Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.Authors - Yanpu Chen | ||
|
Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2Authors - Jin Wang | ||
|
Sci Transl Med MG53-mediated cell membrane repair protects against acute kidney injury.Authors - Pu Duann | ||
|
ACS Nano Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.Authors - Han Lin | ||
|
Nat Commun TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrityAuthors - Sujin Kang | ||
|
Nat Commun UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesisAuthors - Jeonghyun Kim |
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
。我们在这里介绍收集到的一些经验和最佳实践，建立可重复的工作流程的四个关键步骤，包括抗体组合开发、染色、图像采集和分析，可节省研究人员的时间，并最终提高药物发现和开发的效率。 1. 建立最佳抗体组合 使用 Opal 多重荧光免疫组化工作流程准确、可重复地鉴定组织中的不同细胞群及其关系，需要仔细设计、开发和优化用于多重生物标志物定量的抗体组合[1]。 a 从预先设计的抗体组合开始 要选择在多重荧光免疫组化中具有高灵敏度、特异性、再现性和优异性能的可识别所需靶标的抗体似乎并不那么容易
ronaldo_zj 请教各位高手，小弟在实验中发现beta-catenin在细胞核内表达增高，但按照事先的推测，处理了以后会下调该基因的表达，所以有点反常。就想请教一下关于核内beta-catenin增高或者说转位都见于什么情况？考虑何种情况？ 非常感谢各位。 willcloner 是用的什么方法？提取核蛋白，western？还是免疫组化？ 免疫组化有时的结果不是很可靠。 核内beta
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