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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
一年
- 供应商:
上海研卉生物科技有限公司
- 抗体英文名:
ERBB2 Antibody
- 库存:
大量
- 规格:
100ul
Application
|
WB, FC, IHC-P, E |
|---|---|
| Primary Accession | P04626 |
| Other Accession | P06494, P70424 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Other Names | Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL |
|---|---|
| Target/Specificity | This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human ERBB2. |
| Dilution | WB~~1:1000 FC~~1:10~50 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ERBB2 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
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文献和实验上皮及间皮类常用标记抗体EPITHELIAL AND MESOTHELIAL MARKERSCK (MNF 116)- MNF-116 is a pan-cytokeratin antibody.- Source: Dakopatts.- Dilution 1:50.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically
多是多克隆;而小鼠来源的抗体多是单克隆,但也有另外。这条主要要与后面的二抗来源相匹配。(3)实验目的是检测什么种属的抗原,即species reactivity。这一点很重要,一般说明书上都有注明,如小鼠Ms、大鼠Rat、人Hum等等。(4)能否做免疫组化。一般一抗说明书都会注明做WB、IHC、ICC、IF等,建议最好选择注明的抗体,因为一般都是经过文章证明。(5)检测标本类型。用于检测石蜡切片还是冰冻切片,一般能做石蜡切片的抗体,可能都可以用来检测冰冻切片,但能做冰冻切片的抗体,不一定能检测石蜡
肺部肿瘤常用免疫组化抗体: CK7 细胞角蛋白7 阳性部位:胞浆 细胞角蛋白7(CK7)分子量为54KDa,主要标记腺上皮和移行上皮,卵巢,肺和乳腺上皮CK7阳性,而结肠、前列腺和胃肠道上皮为CK7阴性,因此可用于肺癌(CK7+)与结肠癌(CK7-)的鉴别。 CK20 细胞角蛋白20 阳性部位:胞浆 细胞角蛋白20(CK20)的分子量为46KDa,主要标记胃肠道上皮、尿道
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