雌激素受体,ANTI-ER

雌激素受体,ANTI-ER

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  • ¥1200
  • affinity
  • AF6058
  • 中国
  • 2025年08月16日
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    • 详细信息
    • 询价记录
    • 技术资料
    • 库存

      大量

    • 供应商

      上海研卉生物科技有限公司

    • 保存条件

      低温

    • 保质期

      一年

    • 抗体英文名

      Estrogen Receptor-alpha Antibody

    • 规格

      50ul

    雌激素受体,ANTI-ER

    名 称:
    Estrogen Receptor-alpha 抗体
    货 号:
    AF6058
    来 源:
    Rabbit
    应 用:
    WB,IHC,IF/ICC,ELISA
    反 应:
    Human, Mouse, Rat
    预 测:
    Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
    蛋白号:
    P03372
    分子量:
    66kDa
    浓 度:
    1mg/ml
    Alternative Names:Expand▼

    DKFZp686N23123; ER alpha; ER; ER-alpha; Era; ESR; ESR1; ESR1_HUMAN; ESRA; Estradiol receptor; Estrogen nuclear receptor alpha; Estrogen receptor 1; Estrogen receptor alpha 3*,4,5,6,7*/822 isoform; Estrogen receptor alpha; Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform; Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform; Estrogen receptor alpha delta 4 +49 isoform; Estrogen receptor alpha delta 4*,5,6,7*/654 isoform; Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform; Estrogen receptor alpha E1 E2 1 2; Estrogen receptor alpha E1 N2 E2 1 2; Estrogen receptor; ESTRR; NR3A1; Nuclear receptor subfamily 3 group A member 1;

    Applications:

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Reactivity:

    Human, Mouse, Rat

    Predicted Reactivity:

    Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

    Source:

    Rabbit

    Clonality:

    Polyclonal

    Purification:

    The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

    Specificity:

    Estrogen Receptor-alpha Antibody detects endogenous levels of total Estrogen Receptor-alpha.

    Format:

    Liquid

    Concentration:

    1mg/ml

    Storage Condition and Buffer:

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

    AF6058 Estrogen Receptor-alpha Antibody IHC Human breast cancer
    AF6058 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
    AF6058 Estrogen Receptor-alpha Antibody IF/ICC 293
    AF6058 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
    AF6058 Estrogen Receptor-alpha Antibody IF/ICC MCF-7
    AF6058 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

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