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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Screening lambda cDNA or genomic libraries
"NZY" plates for plating lambda libraries.In practice,LB medium works fine.Bacterial host strain- For most lambda strains use RY1073 or C600 HflA or LE392 for lambda hosts.Actually almost any e coli (like TG1)are ok for some lamda vectors,like lambda
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
because of the known antiviral effects of this cytokine as well as its correlation to the cytotoxic capacity of T cells. However, maintenance of both IFN- and IL-2 secretion by pathogen-specific T cells has been linked to a more favorable clinical outcome in human
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