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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Eccles:Protein Lysates from Tissue
load 7.5uL of protein lysate per well so aliquot slightly more than this to each tube to completely avoid any freeze/thawing of samples (causes protein degradation)) Quantitate protein content of lysate (we currently use the BCA kit from Pierce
【共享】经验:The RNeasy Mini Kit from Qiagen
, one of the easiest methods is the QIAshredder, a companion product for the Mini Kit but part of the plant kit. Lysates are loaded into QIAshredders that are then microfuged for 2 minutes to mechanically homogenize the samples. All these options
