IL-1 alpha ELISA Kit (for Lysa

Use of B-PER lysis reagent to purify recombinant proteins tagged with His6 or maltose binding protein

temperature,by which time the pellets were uniformly dispersed in the lysis solution.From this point,the IPIII-His and MBP fusion lysates were processed differently,as detailed in the next two paragraphs. Purification of His-tagged fusion proteins

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

or HP1s associated with 5' biotin labeled siRNA EF52. Schematic methodology is shown for detection from cell lysates of biotin labeled siRNAs in complex with flag-tagged DNMTs or HP1s (alpha, beta, or gamma). B) Triple immunoprecipitation assay

人造血干细胞扩增的研究进展

少。 　　2.1.2 细胞因子的造血调节根据细胞因子对造血细胞不同的作用阶段将其分为：⑴特异性细胞因子：大多作用于分化后期，包括EPo、TPo、M-CSF、G-CSF和IL-5；⑵无系特异性细胞因子：作用于分化状态的HSC，包括IL-3、GM-CSF和IL-4，其功能主要维持处于G0期之外所有HSC的生存、增生；⑶G0期作用细胞因子：包括IL-1、IL-3、IL-6、IL-11、IL-12、G-CSF、LIF和SCF，维持早期HSC的存活或促其分化扩增：⑷抑制血细胞生成的细胞因子：包括