Matrices listed below were spiked with certain level of IL-1alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
95
EDTA plasma(n=5)
88-98
94
heparin plasma(n=5)
89-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.