CCL-97 R2C 大鼠睾丸

间质细胞瘤

CCL-97 R2C 大鼠睾丸

间质细胞瘤

询价

价格询价
品牌 ATCC
货号 R2C
更新日期 2024年10月25日

上海复祥生物科技有限公司

12 年金牌会员

供应商：
复祥生物
物种来源：
鼠
是否是肿瘤细胞：
是
库存：
大量

CCL-97 R2C 大鼠睾丸间质细胞瘤，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

CCL-97 R2C 大鼠睾丸间质细胞瘤的详细介绍

CCL-97 R2C 大鼠睾丸间质细胞瘤

ATCC^® Number:

CCL-97™

Price:

$438.00

Designations:	R2C
Depositors:	G Sato
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Rattus norvegicus (rat)
Morphology:	epithelial
Source:	Strain: WFu Organ: testis Disease: Leydig cell tumor
Cellular Products:	steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host (Roche FuGENE® Transfection Reagents)
Virus Resistance:	poliovirus 2
Reverse Transcript:	positive
Age:	2 months
Gender:	male
Comments:	Stimulation by other hormones or cAMP is not required for hormone secretion.
Propagation:	ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% horse serum to a final concentration of 15% Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Add fresh medium at least twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040
References:	1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022

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CCL-97 R2C 大鼠睾丸

CCL-97 R2C 大鼠睾丸间质细胞瘤，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

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