NRK-49F 大鼠正常肾成纤维细胞

NRK-49F 大鼠正常肾成纤维细胞

EMEM+10FBS。细胞货期8-10个工作日

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NRK-49F 大鼠正常肾成纤维细胞 的详细介绍

ATCC® Number:	CRL-1570™	Price:	$0.00
Designations: NRK-49F Depositors: JE DeLarco Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Rattus norvegicus (rat) Morphology: fibroblast Source: Organ: kidney Disease: normal Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Applications: transfection host (Roche FuGENE® Transfection Reagents) Receptors: epidermal growth factor (EGF); multiplication stimulating activity (MSA) Comments: NRK-49F cells are contact inhibited and very sensitive to transformation by chemicals and viruses. Cultures should be subcultured before they become confluent. If allowed to become confluent, the cells will transform. Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5% Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Preservation: culture medium 95%; DMSO, 5% Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 References: 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450 23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742 32442: Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610 32705: Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987 33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609 33159: Centrella M, et al. Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513