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文献和实验Introduction on Assessments for Spinal Cord Injury
This chapter introduces the chapters of Part VIII, which describe a variety of assessment methods to determine the outcomes after spinal cord injury (SCI) in animal models. The SCI community should be encouraged by the view that SCI
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
Living specimens often do not survive long in the heat from an intense microscope illuminator bulb, usually because the specimen dries up. This problem is easily solved by preparing a vaseline chamber. Simply take a single cover slip and hold
The ribonuclease protection assay (RPA)
for each RPA setup. With the Pharmingen control RNA, 2 µl volume (i.e., 2 µg) is recommended. 10. If RNA has been stored in water, freeze the samples for 15 minutes at -70°C. Dry completely (~1 hour) in a vacuum evaporator centrifuge (no heat
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