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文献和实验in at least one plasmid without mutations. Materials Thermocycler PCR tubes Primers Phusion PCR Mastermix(or another high fidelity polymerase mastermix) BioBrick template DNA (miniprepped plasmid diluted 1:1000 in water) Qiagen PCR
of contamination associated with manual processes. However, preparing material for early clinical trials can be problematic because the batch size is normally too small for a filling machine (e.g., 500-1000 vials). Normal practice is to fill this number of vials
Imaging Plasma Membrane Proteins by Confocal Microscopy
Confocal microscopy has become a widely used method in the study of plasma membrane proteins. A Medline search for the terms “plasma membrane” and “confocal” returns over 1300 references since 1966. Of these, over 1000 references appeared
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