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文献和实验High Cell-Density Fermentation
(AOX1 ) promoter for high-level expression of foreign genes. The AOX1 promoter is induced by methanol, but repressed in the presence of excess glycerol. Glycerol is readily metabolized by P. pastoris , and high-cell densities can be obtained utilizing
Overgo Probing of High-Density Filters
I have been using double-stranded 36mers form STS to probe high-density PAC/BAC filters. The single-stranded overhangs (14 bases) are filled in with 32 P labelled ATP and CTP. POsitive clones are then fingerprinted by complete restriction enzyme
Photolithographic Synthesis of High-Density Oligonucleotide Arrays
High-density polynucleotide probe arrays provide a massively parallel approach to genetic sequence analysis that is having a major impact on biomedical research and clinical diagnostics (1 ). These arrays are comprised of large sets
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