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- 详细信息
- 文献和实验
- 技术资料
- 库存:
200g
- 保质期:
10年
- 供应商:
天府新区正龙生化制品研究室
- 规格:
25g/瓶
真正Pharmacia进口分装和原装G-100。
粒度:40~120微米,
床容积/克干胶:15~20ml;
分离范围:1,000~100,000;
用途:经典的分子筛介质,用于蛋白质的纯化和分离、分子量的测定。
提供原厂说明书。有偿提供技术支持。
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文献和实验Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing
Sephadex G-50 spun column purificati
the syringe barrel into a 10ml centrifuge tube and spin at 3,000g for 5 minutes in a MSE Centaur with swingout rotor. 3) After centrifugation, 100-200ul of the required buffer is added to the top of the column and the column re-centrifuged. Repeat 3 times
Sephadex G-50 spun column purificati
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1ml syringe
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