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500 μL
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文献和实验Denaturing Agarose Gel Electrophoresis of RNA
, such as those from needle biopsies or from laser capture microdissected samples, result in very low yields. In these cases, it may be impossible to spare 200 ng of RNA to assess integrity. Alternative nucleic acid stains, such as SYBR® Gold and SYBR® Green II RNA gel stain
ii. 1:10000 dilution of SYBR® Green II RNA Gel Stain (cat. no. S7564) in TBE for 20-40 minutes. No destaining is required. 7) Visualize ladder bands on a UV transilluminator (302 nm) and photograph using a gel documentation
【转载】protocol for real-time PCR
1 ml 4. Add the reaction mixture to the RNA/primer mixture, mix briefly, and then p lace at room temperature for 2 min. 5. Add 1 ml (50 units) of SuperScript II RT to each tube, mix and incubate at 25°C for 10 min. 6. Incubate the tubes at 42°C
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