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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 抗体英文名：
mouse CD25 (IL-2Rα)，小鼠体内用CD25抗体
- 抗体名：
mouse CD25 (IL-2Rα)，小鼠体内用CD25抗体
- 保存条件：
低温
- 规格：
25mg
About InVivoPlus anti-mouse CD25 (IL-2Rα)
The PC-61.5.3 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The PC-61.5.3 antibody has been shown to inhibit the binding of IL-2 to both the low and high affinity IL-2 receptor forms. Additionally, the PC-61.5.3 antibody is commonly used to deplete CD4+FoxP3+ T regulatory cells in vivo.
InVivoPlus anti-mouse CD25 (IL-2Rα) Specifications
| Isotype | Rat IgG1, λ |
| Recommended Isotype Control(s) | InVivoPlus™ rat IgG1 isotype control, anti-horseradish peroxidase |
| Recommended Dilution Buffer | InVivoPure™ pH 7.0 Dilution Buffer |
| Immunogen | IL-2-dependent cytolytic mouse T cell clone B6.1 |
| Reported Applications |
|
| Formulation |
|
| *Endotoxin |
|
| *Aggregation |
|
| Purity |
|
| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein A High Salt |
| RRID | AB_1107619 |
| Molecular Weight | 150 kDa |
| *Murine Pathogen Test Results |
|
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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文献和实验AUTOMACS系统自动分选体系分选小鼠CD4+ CD25+抑制性细胞
PriCells: AUTOMACS 系统自动分选体系分选 小鼠CD4+ CD25+ 抑制性细胞 实验材料： 1. 小鼠 2. HBSS洗涤缓冲液 3. MACS缓冲液 4. CD8a（Ly-2）磁珠 5. 结合缓冲液 6. biotin-抗CD25（7D4） 7. Streptavidin-PE 8. 抗
CD25 分子 CD25 常用单克隆抗体或代号： TAC,7G7/B6;(IL- 2Ra ) 主要表达细胞： Pre- T,Ta,Ba,Ma [CR] 分子质量(kDa)和结构： gp55(含2个CCP结构域) 功 能： 组成高亲力IL- 2受体,T细胞生长 CD25 Aka IL-2 receptor alpha chain, exists in at least 3 forms
实验材料： 1. 小鼠 2. HBSS洗涤缓冲液 3. MACS缓冲液 4. CD8a（Ly-2）磁珠 5. 结合缓冲液 6. biotin-抗CD25（7D4） 7. Streptavidin-PE 8. 抗PE磁珠 9. 完全RPMI培养基 10. 小鼠CD3+ T细胞富集柱和1×纯化柱缓冲液 11. 15ml离心管，无菌 12. autoMACS系统和分选柱
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