ZR-Duet DNA/RNA MiniPrep

Plasmid Miniprep

with 1 volume of chloroform x1, spin 2 min. Transfer aqueous phase to a new tube. 9) Add 1 volume of isopropanol, mix. Spin at max. speed x 2 min. 10) Rinse with 70% ethanol. Dry pellet. Resuspend in 80 µL of T.E.   Optional (for DNA sequencing) 11) Add 20

Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.

DNA Plasmid Miniprep Protocol

in 0.4 ml of TE buffer (10 mM Tris-Cl, 1 mM EDTA, pH 7.5). Add 10ul of RNAse A solution (20 mg/ml stock stored at -20 ℃), vortex and incubate at 37 ℃ for 20 to 30 minutes to digest remaining RNA. 8. Extract proteins from the plasmid DNA using PCIA