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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温/低温
- 保质期:
说明书
- 英文名:
Mouse 5-hmC & 5-mC DNA Set
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- 规格:
1set
名称:Mouse 5-hmC & 5-mC DNA Set
货号:D5019
规格:1set
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
of TE by scraping on rack and vortexing 15 min. 9. Restriction Enzyme Digest Premix: 4l Bam H1, 4 l 10X buffer, 0.4 l BSA /sample Aliquot 8.4 l / third set of tubes Add 31.6 l genomic DNA and pipet
DNA preparation from mouse tails
General precautions to be followed during handling of any genomic DNA sample: Avoid Shearing of the DNA by limiting mixing to inversion and gentle shaking. Vortexing of samples is not required to perform the following protocols
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