Femto Fungal DNA Quantificatio

Fungal Midi DNA Kit Protocol for Fresh/Frozen Specimens

(not supplied with this kit). Apply 500 ul Elution Buffer (or sterile deionized water) pre-warmed to 65°C and incubate at room temperature for 3 min. Centrifuge at 8,000 x g for 10 min to elute DNA. Smaller volumes will significantly increase DNA concentration

Detection and Quantification of Fetal DNA in Maternal Plasma by Using LightCycler Technology

Since the demonstration of cell-free fetal DNA in maternal circulation, its aberrational quantification has been explored and demonstrated in numerous clinical situations. Moreover, several centers have begun to use detection

The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses

Here, we describe methods that allow the quantification of the ryegrass Neotyphodium lolii fungal endosymbiont and major alkaloids in its host plant Lolium perenne . Fungal concentrations were quantified in total genomic DNA (g DNA) isolated from infected plant tissues