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源叶现货试剂/标准品,库存足,周末发货Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
上海烜雅生物科技有限公司
- 肿瘤类型:
人非小细胞肺腺癌细胞_NCI-H1975
- 细胞类型:
科研细胞
- ATCC Number:
/
- 品系:
人非小细胞肺腺癌细胞_NCI-H1975
- 组织来源:
人非小细胞肺腺癌细胞_NCI-H1975
- 相关疾病:
腺癌;非小细胞肺癌
- 物种来源:
鼠/人/其它
- 免疫类型:
人非小细胞肺腺癌细胞_NCI-H1975
- 细胞形态:
上皮细胞样 贴壁生长
- 是否是肿瘤细胞:
/
- 器官来源:
肺
- 运输方式:
常温运输/干冰运输
- 年限:
尽快解冻复苏细胞进行培养
- 生长状态:
贴壁/悬浮
- 英文名:
NCI-H1975
- 规格:
1x10^6
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:



(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人非小细胞肺腺癌细胞_NCI-H1975相关产品:
| EL-4 | 鼠T淋巴细胞瘤 |
| FOX-NY | 小鼠淋巴瘤细胞 |
| GH3 | 大鼠垂体瘤细胞 |
| GT1.1 | 小鼠垂体瘤细胞(分泌促生长激素分泌激素) |
| HEPA1-6 | 小鼠肝癌细胞 |
| MEL | 小鼠红白血病细胞 |
| MFC | 小鼠前胃癌细胞 |
| MMQ | 大鼠垂体瘤细胞 |
| NG108-15 | 小鼠神经细胞瘤/大鼠神经胶质细胞瘤杂交瘤细胞 |
| P19 | 小鼠畸胎瘤细胞 |
| PC-12 | 大鼠嗜铬细胞瘤细胞 |
| RAW264.7 | 小鼠巨噬细胞瘤 |
| SP2/0-AG14 | 小鼠骨髓瘤 |
| Yac-1 | 小鼠淋巴瘤细胞 |
| H-4-II-E | 大鼠肝细胞瘤 |
| L1210 | 小鼠淋巴细胞白血病 |
| RBL-1 | 大鼠嗜碱性粒细胞白血病 |
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文献和实验一、实验材料NCI-H1299,购自上海晶莱生物技术有限公司。表 2.1.1 主要试剂试剂与耗材厂家(货号)细胞培养瓶FALCON 中国(353014)Penicillin/streptomycin solution(KGY002)0.25% Tripsin-EDTA(BK-E3076)PBS(BK330-2)胎牛血清GIBCO 美国(10270-106)1640 培养基Hyclone 美国(SH3080901)Annexin V-FITC/PI 凋亡试剂盒 二、实验仪器表 2.2.1 主要
potential loss and cell cycle arrest》的论文。文章了评估 epicatechin 对 NCI-H2172 非小型细胞肺癌细胞的抗癌作用,重点观测其对自噬细胞死亡、线粒体膜电位 (m) 丧失和细胞周期阻滞的影响。图片来源于 Molecular Medicine Reports 官网然而,就在发表后的第二天,PubPeer 网站上的匿名网友指出,文章 Fig4 中的多个细胞为复制细胞。匿名网友指出,相同颜色圈出的细胞是相同的细胞。图片来源于 PubPeer 官网,相同圈中细胞
MDA-MB-157 人乳腺癌细胞 MDA-MB-231 人乳腺癌细胞 MDA-MB-435s 人乳腺癌细胞 MDA-MB-453 人乳腺癌细胞 MG-63 人骨肉瘤细胞 Mo7e 人巨细胞白血病细胞(B类) MOLT-4 人淋巴细胞白血病细胞 NCI-H157 人非小细胞肺腺癌 NCI-H209 人小细胞肺癌 NCI-H446 人小细胞肺癌 NTERA-2 人恶性多发性畸胎瘤细胞 PA-1 人卵巢畸胎瘤细胞 ANC-1 人胰腺癌细胞 PC-3
技术资料









