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- XY-XB-2128
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- 2025年11月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
上海烜雅生物科技有限公司
- 肿瘤类型：
人肺鳞癌细胞_NCI-H520
- 细胞类型：
科研细胞
- ATCC Number：
/
- 品系：
人肺鳞癌细胞_NCI-H520
- 组织来源：
人肺鳞癌细胞_NCI-H520
- 相关疾病：
人肺鳞癌细胞_NCI-H520
- 物种来源：
鼠/人/其它
- 免疫类型：
人肺鳞癌细胞_NCI-H520
- 细胞形态：
贴壁，上皮细胞样，多角形，紧密排列
- 是否是肿瘤细胞：
/
- 器官来源：
人肺鳞癌细胞_NCI-H520
- 运输方式：
常温运输/干冰运输
- 年限：
尽快解冻复苏细胞进行培养
- 生长状态：
贴壁/悬浮
- 英文名：
NCI-H520
- 规格：
1x10^6
产品简介：
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全：
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装：
提供新鲜或者冻存的细胞
使用方法：
如是新鲜细胞，客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时，再进行后续的实验操作；如是冻存细胞， 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 ：
（1）Getting Started with an ATCC Cell Line：
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
（2）细胞图片：
（3）Initiating Frozen Cultures：
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠，售后有保障
如运输过程中导致细胞污染或者死亡，我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人肺鳞癌细胞_NCI-H520相关产品：
| SF126 | 人脑瘤 |
| SF17 | 人脑瘤 |
| SF17 | 人脑瘤 |
| SF763 | 人脑瘤 |
| SF767 | 人脑瘤 |
| SH-SY5Y | 人骨髓神经母细胞瘤 |
| SK-BR-3 | 人乳腺癌细胞 |
| SK-MEL-1 | 人皮肤黑色素瘤细胞 |
| SK-N-SH | 人神经母细胞瘤 |
| SK-OV-3 | 人卵巢腺瘤细胞 |
| SMMC-7721 | 人肝癌细胞 |
| SW13 | 人肾上腺皮质瘤 |
| SW480 | 人结直肠癌 |
| T84 | 人结肠癌细胞 |
| THP1 | 人单核细胞型淋巴瘤 |
| U-2 OS | 人骨肉瘤细胞 |
| U251 | 人神经胶质细胞瘤 |
| U-937 | 人淋巴瘤细胞 |
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文献和实验都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
双向凝胶电泳-飞行时间质谱法分析人肺鳞癌细胞HCL-H520蛋白质组成份
《双向凝胶电泳-飞行时间质谱法分析人肺鳞癌细胞HCL-H520蛋白质组成份》下载 点击 这里 下载
A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
, are RARB2 -transfectants that were established in our laboratory (21 ). MM-1 was also established in our laboratory (6 ). NCI-H23, NCI-H82, NCI-H125, NCI-H157, NCI-H520, and NCI-H596 were supplied by Dr. Adi Gazdar (NCI, NIH, Bethesda, MD). NBE-E6 E7 (22
技术资料
