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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 细胞类型:
科研
- 品系:
详情请咨询我司客服
- 组织来源:
详情请咨询我司客服
- 物种来源:
鼠/人/其它
- 细胞形态:
上皮样/成纤维样/其它
- 器官来源:
详情请咨询我司客服
- 运输方式:
常温运输/干冰运输
- 年限:
详情请咨询我司客服
- 生长状态:
贴壁/悬浮
- 英文名:
HNE2-LMP1/2
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:

(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人鼻咽癌细胞系_HNE2-LMP1/2相关产品:
| CNE(鼻咽癌细胞) |
| RT4(膀胱癌细胞) |
| J82(膀胱癌细胞) |
| SHG44(胶质瘤细胞) |
| LoVo(人结肠癌细胞) |
| V79(中国仓鼠肺细胞) |
| U937(组织细胞淋巴瘤) |
| PT-67(病毒转染小鼠细胞) |
| HEL(红白血病细胞) |
| 293(转染腺病毒E1A基因的人肾上皮细胞) |
| C6(脑胶质瘤细胞) |
| SHZ-88(大鼠乳腺癌细胞) |
| CBRH-7919(大鼠肝癌细胞) |
| NCL-H460(非小细胞肺癌) |
| F9(畸胎瘤细胞) |
| 5637(人膀胱癌细胞) |
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文献和实验)、HeLa(人宫颈癌细胞系)、CNE1-LMP1(稳定表达LMP1的鼻咽癌细胞系)、HNE2(鼻咽癌细胞系)和Tet-on-LMP1-HNE2(受四环素诱导可调控的鼻咽癌细胞系)等7种上皮来源的细胞系的细胞蛋白提取液及培养上清,结果在7种上皮来源的肿瘤细胞系的蛋白提取液及培养上清中,均检测到了人免疫球蛋白A。认为上皮来源的肿瘤细胞可表达免疫球蛋白A[18]。 2002年,Kotaka等用差减杂交方法检测肝细胞癌(HCC)与HCV伴发的关系时,发现与正常肝组织相比,HCC组织中免疫球蛋白λ轻链基因
表达,而已知Il-8能刺激血管发生。了解神经胶质瘤支持血管生长的机制有望发现潜在的新药靶点,包括恢复ING4 功能,通过NF-kappa B抑制Il-8生产、或者阻断肿瘤细胞对Il-8敏感性的药物等。 5.肿瘤抑制基因drs:一种新的凋亡途径 日本Shiga大学医学院的研究人员报告[4],最初分离Drs基因是作为抑制v-src转化过程。drs 基因mRNA表达在一种人类癌细胞系和组织中明显下调,这表明drs 基因可作为肿瘤抑制基因。在本研究中,研究人员发现Drs蛋白的异常表达导致人类癌细胞系凋亡。应用
质、DNA和RNA合成中起作用。锰在自然界分布广泛,以茶叶中含量最丰富。镁的缺乏较少。若吸收过多可出现中毒症状,主要由于生产及生活中防护不善,以粉尘形式进入人体所致。锰是一种原浆毒,可引起慢性神经系统中毒,表现为锥体外系的功能障碍。并可引起眼球集合能力减弱,眼球震颤、睑裂扩大等。 五、硒 硒是人体必需的一种微量元素,体内含量约14-21mg,广泛分布于除脂肪组织以外的所有组织中。主要以含硒蛋白质形式存在。人体每日硒的需要量为50-200μg。 硒是谷胱甘肽过氧化物酶(GSH�x
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