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磷酸化视网膜母细胞瘤相关蛋白1抗体图片

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  • 询价
  • 上海博湖
  • BH-K21580
  • 进口、国产
  • 2025年07月16日
  • WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
  • 详见说明书
  • 详见说明书
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    • 技术资料
    • 抗体名

      磷酸化视网膜母细胞瘤相关蛋白1抗体图片

    • 抗体英文名

      Anti-Phospho-Rb/P105 RB (Thr821)

    • 靶点

      详见说明书

    • 浓度

      1mg/1ml

    • 应用范围

      WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

    • 宿主

      详见说明书

    • 供应商

      上海博湖

    • 库存

      大量

    • 级别

      详见说明书

    • 目录编号

      详见说明书

    • 抗原来源

      Rabbit

    • 保质期

      详见说明书

    • 适应物种

      详见说明书

    • 标记物

      详见说明书

    • 克隆性

      多克隆

    • 保存条件

      Store at -20 °C

    • 形态

      大量

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human Retinoblastoma associated protein around the phosphorylation site of Thr821

    • 规格

      0.2ml/200μg

    磷酸化视网膜母细胞瘤相关蛋白1抗体图片是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品,产品品质卓越,价格实惠,多种规格供应,售后完善。
    英文名称  Anti-Phospho-Rb/P105 RB (Thr821)
    中文名称  磷酸化视网膜母细胞瘤相关蛋白1抗体图片
         OSRC; P105 RB; P105RB; PP105; PP110; pRb; RB 1; RB1; RB1 protein; Retinoblastoma 1 (including osteosarcoma); Retinoblastoma 1; Retinoblastoma associated protein; Retinoblastoma related osteosarcoma; Retinoblastoma susceptibility gene; Including osteosarcoma; RB_HUMAN.

    产品细节图片1
         1mg/1ml
     0.1ml/100μg
    抗体来源  Rabbit
    克隆类型  polyclonal
    交叉反应  Human, Mouse, Rat, Pig, Cow, Horse, Rabbit
    产品类型  一抗 磷酸化抗体  
    研究领域  肿瘤 细胞生物 免疫学 信号转导 转录调节因子
    蛋白分子量  predicted molecular weight: 102kDa 
         Lyophilized or Liquid
     KLH conjugated Synthesised phosphopeptide derived from human Retinoblastoma associated protein around the phosphorylation site of Thr821
         IgG
    纯化方法   affinity purified by Protein A
     0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

    产品细节图片2

    磷酸化视网膜母细胞瘤相关蛋白1抗体图片产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
    (石蜡切片需做抗原修复) 
     not yet tested in other applications.
     optimal dilutions/concentrations should be determined by the end user.  
    保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
    Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
    产品介绍 Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

    Function : Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
    Subunit : Interacts with ATAD5. Interacts with PRMT2, CDK1 and CDK2. The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. Interacts with heterodimeric E2F/DP transcription factor complexes containing TFDP1 and either E2F1, E2F3, E2F4 or E2F5, or TFDP2 and E2F4. The unphosphorylated form interacts with EID1, ARID3B, KDM5A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with NDC80. Interacts with GRIP1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1 and LIMD1. Interacts with SMARCA4/BRG1 AND HDAC1. Interacts with adenovirus E1A protein, HPV E7 protein and SV40 large T antigen. Interacts with PSMA3 and USP4. Interacts (when methylated at Lys-860) with L3MBTL1.
    Subcellular Location : Nucleus.
    Tissue Specificity : Expressed in the retina.
    Post-translational modifications : Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1. Monomethylated at Lys-860 by SMYD2, promoting interaction with L3MBTL1.
    DISEASE : Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ('cat eye') is investigated.
    Defects in RB1 are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
    Defects in RB1 are a cause of osteogenic sarcoma (OSRC) [MIM:259500].
    Similarity : Belongs to the retinoblastoma protein (RB) family.
    Database links : UniProtKB/Swiss-Prot: P06400.2

    产品细节图片3
    磷酸化视网膜母细胞瘤相关蛋白1抗体图片具有全、新、优、品、好四大特点:
    全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
    新:产品更新速度较快,基本上每周都有新产品出现。
    优:产品质量好,投诉比较少。
    好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
    我们提供的标记服务,可根据您科研的需求,满足您的需要,质量保证,价格合理。
    一、标记流程
    委托标记服务,请下载并详细填写《委托标记申请单》,待我公司标记室技术人员研究、确认后答复,并办理委托标记事宜。
    二、各种标记物制备的收费标准(RMB 
    我们不但提供抗体和大分子蛋白的标记服务,还提供小分子多肽及小分子蛋白的各种标记服务,提供标记化合物的服务(此化合物必须适用于标记),价格另议。
    一抗和二坑的区别:
    第一抗体就是平常所说的抗体,即能和抗原特异性结合。
    第二抗体是能和抗体结合的,即抗体的抗体。主要用于检测抗体的存在。
    一抗是针对抗原的抗体,二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说,抗原进入机体刺激机体免疫系统产生免疫应答,由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
    一抗二抗都是一种可以特异结合别的物质的基团,而且一抗可以至少结合两种其他基团(底物和二抗)。
    一抗:可以特异结合底物,就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
    二抗:可以和一抗结合,并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团),则不需要二抗。但这样成本很高,因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记,再来检测一抗。而一抗识别底物。这样,当一抗结合到底物上,就可以通过二抗检测出来。 

    产品细节图片4
    Anti-RGS2/FITC 荧光素标记G蛋白信号转导调节因子2抗体IgGMulti-class antibodies规格: 0.2ml
    IGF-I( Insulin-like Growth Factor-I ) 胰岛素样生长因子-I(抗原)Multi-class antibodies规格: 0.5mg
    髓鞘相关糖蛋白a/b抗体 Anti-MAG-a/b 0.1ml
    Sulfatase 2 英文名称: 酯酶2蛋白抗体 0.2ml
    Fbx15 英文名称: Fbx15蛋白抗体 0.2ml
    Rhesus antibody Rh phospho-PKA R2 (Ser96) 化蛋白激酶A受体2α亚基抗体 规格 0.1ml
    IGF-I( Insulin-like Growth Factor-I ) 胰岛素样生长因子-I(抗原)Multi-class antibodies规格: 0.5mg
    GFP (Green Fluorecent protein) 绿色荧光蛋白(多肽抗原)Multi-class antibodies规格: 0.5mg

    Anti-CD146/MCAM 黑色素瘤细胞粘附分子CD146抗体Multi-class antibodies规格: 0.2ml
    Rhesus antibody Rh PARP16 多腺苷二多聚酶16抗体 规格 0.2ml
    HA Kit Influenza 甲型流感 H4N6 (A/mallard/Ohio/657/2002) 血凝素 (Hemagglutinin / HA) ELISA配对抗体 ELISA
    TRPC1 英文名称: 瞬时受体电位通道1抗体 0.1ml
    ChRM2 英文名称: 毒蕈碱型乙酰胆碱受体M2抗体 0.1ml
    Anti-CD146/MCAM 黑色素瘤细胞粘附分子CD146抗体Multi-class antibodies规格: 0.2ml
    SLC14A1 英文名称: 尿素转运型糖蛋白抗体 0.2ml

    ENPP5 英文名称: /二酯酶家族成员5抗体 0.2ml
    神经肽Y1受体抗体 Anti-NPY1R 0.1ml
    Anti-Slc22A17/FITC 荧光素标记可溶性载质转运蛋白22A17抗体IgGMulti-class antibodies规格: 0.2ml
    Rhesus antibody Rh phospho-P53(Ser33) 化抑制基因P53抗体 规格 0.1ml
    BNP 脑素(多肽抗原)Multi-class antibodies规格: 0.5mg
    小鼠抗小核糖核蛋白/Sm抗体(sNP/Sm)ELISAKit   ELISA.   96T/48T

    小鼠丝酸/苏酸蛋白酶(STK)ELISAKit   ELISA.   96T/48T
    小鼠高铁血红蛋白(MHB)ELISAKit   ELISA.   96T/48T
    小鼠骨退化特异标志物(CTX-2)ELISAKit   ELISA.   96T/48T
    大鼠胰岛素样蛋白3(INSL3)ELISA试剂盒 ,英文名: INSL3 ELISA Kit

    Mouse urokinase type plasminogen activator (uPA) ELISA Kit 小鼠尿激酶型纤溶酶原激活物(uPA)ELISA试剂盒
    Mouseansforminggrowthfactorα,TGF-αELISAKit 小鼠转化生长因子α(TGF-α)ELISA试剂盒 96T/48T 进口分装
    CLIAKitforHumanCoisolELISAKit人皮质醇规格:48T/96T
    细胞BMK激酶活性定量检测试剂盒(A/B/C)20
    ELISAKitBMP-7大鼠骨成型蛋白7规格:48T/96T
    磷酸化视网膜母细胞瘤相关蛋白1抗体图片小鼠甘油-3-脱酶(GAPDH)ELISA试剂盒 ,英文名: GAPDH ELISA Kit

    人肺耐药蛋白(LRP)ELISA检测试剂盒HumanLungresistance-relatedprotein,LRPELISAKit 96T/48T
    大鼠上皮中性粒细胞活化肽78(ENA-78/CXCL5)免疫试剂盒 Rat Epithelial neuophil activating peptide 78,ENA-78 ELISA Kit
    英文名称RatFIXELISAKit大鼠凝血因子IX规格:96T/48T
    活体细胞氧化应激活性氧(ROS)原位染色试剂盒(超氧阴离子)100
    ELISAKitCyclin-D3人细胞周期素D3规格:48T/96T
     

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    图标文献和实验
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      keep-smile 最近在做原代培养的神经细胞的凋亡研究。对于六孔板中蛋白提取有些疑问。药物处理后,总有一些细胞漂浮起来,估计就是凋亡或坏死细胞,直接吸弃培养基怕对凋亡相关蛋白检测有影响。如果细胞消化离心,又担心提取不够迅速使一些目的蛋白去磷酸化,比如MAPK信号通路蛋白。请问大家,应该怎么解决这个问题呢,有什么好的办法? ronaldo_zj 1. 我们也遇到过类似问题,但经过检测,我们发现培养基上清漂浮的细胞确实就是晚期

    • 【求助】如何证明GSK-3beta的抑制是由Wnt通路引起的?

      magichunter 我现在能够证明GSK-3beta的活性受到了抑制,同时也证明了b-catenin在核内和胞浆内的表达都升高了,同时胞浆内的磷酸化b-catenin含量降低(Wnt通路被激活了吗)。另外Akt的磷酸化增高(应该是PI3K/Akt通路被激活了)。因为Wnt和PI3K/Akt两条通路都可以抑制GSK的活性。 请问能否判断GSK的抑制是由Wnt通路的激活引起的,还是有PI3K/Akt的激活引起的?还是两条通路都激活了? (暂时我还不能进行

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