- $875
- LSBio
- To be determined
- LS-F14880
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Dog
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Performance 100% Guaranteed
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Product Description
LS-F14880 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Dog CYCS / Cytochrome c. It is based upon a Custom assay principle.More CYCS / Cytochrome c Products
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Specifications
TypeCustom ELISA kit
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Target
CYCS / Cytochrome c
Synonyms
CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic
Reactivity
Dog
Manual
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
To be determined
Sensitivity
To be determined
Storage
Short term: 4°C; Long term: see manual.
Kit Components
Typical components include:
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Wash Buffer (25x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About CYCS / Cytochrome c
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. P99999 NM_018947 NP_061820.1
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
R&D Systems支原体污染检测新方法（ELISA）专辑
100 mL 和 1000 mL量筒 水平微孔板摇床 涡轮混合器 手套和面具 4. 主要的实验操作流程 A.细胞裂解或细胞上清液准备。若为细胞，终浓度达到1.25x106cells/mL；若为细胞上清，10倍稀释。 B. 预先润洗杂交板。 C. 将“探针”加入杂交板微孔中。 D．将样品、阳性对照、阴性对照加入微孔中，65℃水浴60分钟“杂交”后在洗脱。 E. 预先润洗带有链亲和素的板。 F. 将标记好的样本加入带有链亲和素板
two to three colonies from each agar plate and set upa small volume (typically 5 ml) LB culture for miniprep. Incubate and shake under 37 °C overnight, prepare plasmidDNA using a spin miniprep kit according to manufacturer’sprotocol. 6. (Optional) Digest
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