Mouse IL25 / IL17E ELISA Kit (

Mouse IL25 / IL17E ELISA Kit (Sandwich ELISA)

Product Description

LS-F387 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL25 / IL17E in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL25 / IL17E as low as 49.16 picograms per milliliter.

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IL25 / IL17E
Reactivity
 
Mouse
Specificity
 
The antibody pair provided in this kit recognizes mouse IL25 / IL17E.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
49.16 - 4800 pg/ml
Sensitivity
 
49.16 pg/ml
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Protocol
 

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl standard or sample to each well.
3. Incubate 2.5 hours at room temperature or overnight at 4°C.
4. Add 100 µl prepared Detection Antibody to each well.
5. Incubate 1 hour at room temperature.
6. Add 100 µl prepared Streptavidin solution.
7. Incubate 45 minutes at room temperature.
8. Add 100 µl TMB One-Step Substrate Reagent to each well.
9. Incubate 30 minutes at room temperature.
10. Add 50 µl Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IL25 / IL17E
 
Uniprot: Q9H293 NCBI: NM_022789 NP_073626.1