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- Invent
- NT-032
- 美国
- 2026年01月02日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues
- 保存条件：
4℃
- 规格：
20 Preps
MinuteTM 新鲜/冷冻组织胞质胞核分离试剂盒
目录号：NT-032
描述
从细胞和组织中分离细胞质和细胞核组分是一种常见的实验。细胞样品的组分分离相对简单直接，然而，从组织样品中特别是冷冻组织中将胞质和胞核组分分离干净是很具挑战的。因为冷冻和反复冻融会引起组织细胞结构的改变，加之不适当的匀浆方式和效率低的提取缓冲液，使得交叉污染十分严重。目前大多数商业试剂盒设计可同时用于细胞和组织样品，但是忽略了两种类型样品之间的结构差异。因此，从新鲜/冷冻组织中分离胞质和胞核组分时，交叉污染问题无法避免(见下文参考文献1和2)。这款新型的胞质和胞核分离试剂盒专用于组织样品的胞质胞核分离，试剂盒特有的缓冲液体系和独特的操作方法可以将交叉污染降到最低。整个操作可以在30分钟内完成。
应用：
可应用于SDS-PAGE，immunoblottings，ELISA，IP，蛋白定位，凝胶迁移分析，2-D等其他应用。
Protocol：
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参考文献：
1.Li, Y., Wu, J., Yu, S., Zhu, J., Zhou, Y., Wang, P., ... & Zhao, Y. (2020). Sestrin2 promotes angiogenesis to alleviate brain injury by activating Nrf2 through regulating the interaction between p62 and Keap1 following photothrombotic stroke in rats.Brain Research, 146948.
2.Wang, B., Huang, X., Pan, X., Zhang, T., Hou, C., Su, W. J., ... & Wang, Y. X. (2020). Minocycline prevents the depressive-like behavior through inhibiting the release of HMGB1 from microglia and neurons.Brain, Behavior, and Immunity.
3.Song T, Guan X, Wang X,et al. Dynamic modulation of gut microbiota improves post-myocardial infarct tissue repair in rats via butyric acid-mediated histone deacetylase inhibition.The FASEB Journal. 2021;35:e21385.
4.Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., ... & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.
5.Bao, J., Lu, Y., She, Q. Y., Dou, W., Tang, R., Xu, X., ... & Zheng, C. (2021). MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease. JCI insight.
6.Zhou, Y., Nie, A. Q., Chen, S., Wang, M. M., Yin, R., Tang, B. H., ... & Zhao, W. (2021). Downregulation of Renal MRPs Transporters in Acute Lymphoblastic Leukemia Mediated by the IL-6/STAT3/PXR Signaling Pathway. Journal of Inflammation Research, 14, 2239-2252.
7.Yu, D., Zarate, N., Cuccu, F., Yue, J. S., Brown, T. G., Nanclares, T. N. M., ... & Gomez-Pastor, R. (2021). CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in Huntington’s disease. Acta Neuropathologica Communications (2022) 10:83
8.Weng, J., Wang, X., Xu, B., & Li, W. (2021). Augmenter of liver regeneration ameliorates ischemia-reperfusion injury in steatotic liver via inhibition of the TLR4/NF-κB pathway. Experimental and Therapeutic Medicine, 22(2), 1-8
9.Wang, M., Wang, L., Zhou, Y., Feng, X., Ye, C., & Wang, C. (2021). Icariin attenuates renal fibrosis in chronic kidney disease by inhibiting interleukin-1β/transforming growth factor-β-mediated activation of renal fibroblasts. Phytotherapy Research, 1–12.
10.Yang, C., Luo, P., Chen, S. J., Deng, Z. C., Fu, X. L., Xu, D. N., ... & Liu, W. J. (2021). Resveratrol sustains intestinal barrier integrity, improves antioxidant capacity, and alleviates inflammation in the jejunum of ducks exposed to acute heat stress. Poultry Science, 101459.
11.Imai, S., Ohama, M., Suzuki, M., Katayanagi, Y., Kayashima, Y., & Tezuka, H. Short Alkyl Chain-Length Resorcinol Olivetol Protects Against Obesity with Mitochondrial Activation by Pgc-1α Deacetylation. Available at SSRN 3924601.
12.Wang, D., Wang, T., Li, Z., Guo, Y., & Granato, D. (2022). Green tea polyphenols upregulate the Nrf2 signaling pathway and suppress oxidative stress and inflammation markers in D-galactose-induced liver aging in mice. Frontiers in Nutrition, 130.
13.Lv, L., Shu, H., Mo, X., Tian, Y., Guo, H., & Sun, H. Y. (2022). Activation of the Nrf2 Antioxidant Pathway by Longjing Green Tea Polyphenols in Mice Livers. Natural Product Communications, 17(12), 1934578X221139409.
14.Wahl, D., Smith, M. E., McEntee, C. M., Cavalier, A. N., Osburn, S. C., Burke, S. D., ... & LaRocca, T. J. (2023). The reverse transcriptase inhibitor 3TC protects against age‐related cognitive dysfunction. Aging Cell, e13798.
15.Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.
16.Schmidt, A. V., Monga, S. P., Prochownik, E. V., & Goetzman, E. S. (2023). A Novel Transgenic Mouse Model Implicates Sirt2 as a Promoter of Hepatocellular Carcinoma. International Journal of Molecular Sciences, 24(16), 12618.
17.AlTamimi, J. Z., AlFaris, N. A., Alshammari, G. M., Alagal, R. I., Aljabryn, D. H., & Yahya, M. A. (2023). Esculeoside A (ESA) alleviates reproductive toxicity in streptozotocin-diabetic rats’s model by activating Nrf2 signaling. Saudi Journal of Biological Sciences, 103780.
18.ALTamimi, J. Z., AlFaris, N. A., Alshammari, G. M., Alagal, R. I., Aljabryn, D. H., & Yahya, M. A. (2023). Esculeoside A Decreases Diabetic Cardiomyopathy in Streptozotocin-Treated Rats by Attenuating Oxidative Stress, Inflammation, Fibrosis, and Apoptosis: Impressive Role of Nrf2. Medicina, 59(10), 1830.
19.Al Jadani, J. M., Albadr, N. A., Alshammari, G. M., Almasri, S. A., Alfayez, F. F., & Yahya, M. A. (2023). Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory Effects. Nutrients, 15(22), 4755.
20.Ryan, F., Francos-Quijorna, I., Hernández-Mir, G., Aquino, C., Schlapbach, R., Bradbury, E. J., & David, S. (2023). Tlr4 deletion modulates cytokine and extracellular matrix expression in chronic spinal cord injury, leading to improved secondary damage and functional recovery. Journal of Neuroscience.
21.Alsabaani, N. A., Amawi, K., Eleawa, S. M., Ibrahim, W. N., Aldhaban, W., Alaraj, A. M., ... & Alkh ateeb, M. A. (2024). Nrf-2-dependent antioxidant and anti-inflammatory effects underlie the protective effect of esculeoside A against retinal damage in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy, 173, 116461.
22.Di Veroli, B., Bentanachs, R., Roglans, N., Alegret, M., Giona, L., Profumo, E., ... & Buttari, B. (2024). Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose. Cells, 13(15), 1247.
23.Jawharji, M. T., Alshammari, G. M., Binobead, M. A., Albanyan, N. M., Al-Harbi, L. N., & Yahya, M. A. (2024). Comparative Efficacy of Low-Carbohydrate and Ketogenic Diets on Diabetic Retinopathy and Oxidative Stress in High-Fat Diet-Induced Diabetic Rats. Nutrients, 16(18), 3074.
24.Zhe Zhang; J. Q. Zhang; Rui Shi; Tiantian Xu; Shiduo Wang; et al(2024).Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats.Brazilian Journal of Medical and Biological Research.DOI 10.1590/1414-431x2024e13914
25.Wu T, Jiang F, Wu F, Zheng G, Li Y, Wu L, E2F1 and E2F7 regulate gastric cancer cell proliferation respectively through transcriptional activation and transcriptional repression of MYBL2, Journal of Biological Chemistry (2024), doi: doi.org/10.1016/j.jbc.2024.108027
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- 作者
- 内容
- 询问日期
文献和实验1.Li, Y., Wu, J., Yu, S., Zhu, J., Zhou, Y., Wang, P., ... & Zhao, Y. (2020). Sestrin2 promotes angiogenesis to alleviate brain injury by activating Nrf2 through regulating the interaction between p62 and Keap1 following photothrombotic stroke in rats.Brain Research, 146948.
2.Wang, B., Huang, X., Pan, X., Zhang, T., Hou, C., Su, W. J., ... & Wang, Y. X. (2020). Minocycline prevents the depressive-like behavior through inhibiting the release of HMGB1 from microglia and neurons.Brain, Behavior, and Immunity.
3.Song T, Guan X, Wang X,et al. Dynamic modulation of gut microbiota improves post-myocardial infarct tissue repair in rats via butyric acid-mediated histone deacetylase inhibition.The FASEB Journal. 2021;35:e21385.
4.Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., ... & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.
5.Bao, J., Lu, Y., She, Q. Y., Dou, W., Tang, R., Xu, X., ... & Zheng, C. (2021). MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease. JCI insight.
6.Zhou, Y., Nie, A. Q., Chen, S., Wang, M. M., Yin, R., Tang, B. H., ... & Zhao, W. (2021). Downregulation of Renal MRPs Transporters in Acute Lymphoblastic Leukemia Mediated by the IL-6/STAT3/PXR Signaling Pathway. Journal of Inflammation Research, 14, 2239-2252.
7.Yu, D., Zarate, N., Cuccu, F., Yue, J. S., Brown, T. G., Nanclares, T. N. M., ... & Gomez-Pastor, R. (2021). CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in Huntington’s disease. Acta Neuropathologica Communications (2022) 10:83
8.Weng, J., Wang, X., Xu, B., & Li, W. (2021). Augmenter of liver regeneration ameliorates ischemia-reperfusion injury in steatotic liver via inhibition of the TLR4/NF-κB pathway. Experimental and Therapeutic Medicine, 22(2), 1-8
9.Wang, M., Wang, L., Zhou, Y., Feng, X., Ye, C., & Wang, C. (2021). Icariin attenuates renal fibrosis in chronic kidney disease by inhibiting interleukin-1β/transforming growth factor-β-mediated activation of renal fibroblasts. Phytotherapy Research, 1–12.
10.Yang, C., Luo, P., Chen, S. J., Deng, Z. C., Fu, X. L., Xu, D. N., ... & Liu, W. J. (2021). Resveratrol sustains intestinal barrier integrity, improves antioxidant capacity, and alleviates inflammation in the jejunum of ducks exposed to acute heat stress. Poultry Science, 101459.
11.Imai, S., Ohama, M., Suzuki, M., Katayanagi, Y., Kayashima, Y., & Tezuka, H. Short Alkyl Chain-Length Resorcinol Olivetol Protects Against Obesity with Mitochondrial Activation by Pgc-1α Deacetylation. Available at SSRN 3924601.
12.Wang, D., Wang, T., Li, Z., Guo, Y., & Granato, D. (2022). Green tea polyphenols upregulate the Nrf2 signaling pathway and suppress oxidative stress and inflammation markers in D-galactose-induced liver aging in mice. Frontiers in Nutrition, 130.
13.Lv, L., Shu, H., Mo, X., Tian, Y., Guo, H., & Sun, H. Y. (2022). Activation of the Nrf2 Antioxidant Pathway by Longjing Green Tea Polyphenols in Mice Livers. Natural Product Communications, 17(12), 1934578X221139409.
14.Wahl, D., Smith, M. E., McEntee, C. M., Cavalier, A. N., Osburn, S. C., Burke, S. D., ... & LaRocca, T. J. (2023). The reverse transcriptase inhibitor 3TC protects against age‐related cognitive dysfunction. Aging Cell, e13798.
15.Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.
16.Schmidt, A. V., Monga, S. P., Prochownik, E. V., & Goetzman, E. S. (2023). A Novel Transgenic Mouse Model Implicates Sirt2 as a Promoter of Hepatocellular Carcinoma. International Journal of Molecular Sciences, 24(16), 12618.
17.AlTamimi, J. Z., AlFaris, N. A., Alshammari, G. M., Alagal, R. I., Aljabryn, D. H., & Yahya, M. A. (2023). Esculeoside A (ESA) alleviates reproductive toxicity in streptozotocin-diabetic rats’s model by activating Nrf2 signaling. Saudi Journal of Biological Sciences, 103780.
18.ALTamimi, J. Z., AlFaris, N. A., Alshammari, G. M., Alagal, R. I., Aljabryn, D. H., & Yahya, M. A. (2023). Esculeoside A Decreases Diabetic Cardiomyopathy in Streptozotocin-Treated Rats by Attenuating Oxidative Stress, Inflammation, Fibrosis, and Apoptosis: Impressive Role of Nrf2. Medicina, 59(10), 1830.
19.Al Jadani, J. M., Albadr, N. A., Alshammari, G. M., Almasri, S. A., Alfayez, F. F., & Yahya, M. A. (2023). Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory Effects. Nutrients, 15(22), 4755.
20.Ryan, F., Francos-Quijorna, I., Hernández-Mir, G., Aquino, C., Schlapbach, R., Bradbury, E. J., & David, S. (2023). Tlr4 deletion modulates cytokine and extracellular matrix expression in chronic spinal cord injury, leading to improved secondary damage and functional recovery. Journal of Neuroscience.
21.Alsabaani, N. A., Amawi, K., Eleawa, S. M., Ibrahim, W. N., Aldhaban, W., Alaraj, A. M., ... & Alkh ateeb, M. A. (2024). Nrf-2-dependent antioxidant and anti-inflammatory effects underlie the protective effect of esculeoside A against retinal damage in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy, 173, 116461.
22.Di Veroli, B., Bentanachs, R., Roglans, N., Alegret, M., Giona, L., Profumo, E., ... & Buttari, B. (2024). Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose. Cells, 13(15), 1247.
23.Jawharji, M. T., Alshammari, G. M., Binobead, M. A., Albanyan, N. M., Al-Harbi, L. N., & Yahya, M. A. (2024). Comparative Efficacy of Low-Carbohydrate and Ketogenic Diets on Diabetic Retinopathy and Oxidative Stress in High-Fat Diet-Induced Diabetic Rats. Nutrients, 16(18), 3074.
24.Zhe Zhang; J. Q. Zhang; Rui Shi; Tiantian Xu; Shiduo Wang; et al(2024).Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats.Brazilian Journal of Medical and Biological Research.DOI 10.1590/1414-431x2024e13914
25.Wu T, Jiang F, Wu F, Zheng G, Li Y, Wu L, E2F1 and E2F7 regulate gastric cancer cell proliferation respectively through transcriptional activation and transcriptional repression of MYBL2, Journal of Biological Chemistry (2024), doi: doi.org/10.1016/j.jbc.2024.108027
26.Samuvel DJ, Li L, Krishnasamy Y, Gooz M, Takemoto K, Woster PM, Lemasters JJ, Zhong Z. Mitochondrial depolarization after acute ethanol treatment drives mitophagy in living mice. Autophagy. doi: 10.1080/15548627.2022.2046457.
27.Qiong Mei; Yu Zhang; Hong Li; Wei Ma; Wenkai Huang; et al.(2025).Hepatic factor MANF drives hepatocytes reprogramming by detaining cytosolic CK19 in intrahepatic cholangiocarcinoma.Cell Death and Differentiation.DOI 10.1038/s41418-025-01460-4
28.Fan Zhang; Meng‐Die Li; Fan Pan; Wen‐Jia Lei; Yang Xi; et al.(2025). Nuclear Translocation of S100A9 Triggers Senescence of Human Amnion Fibroblasts by De‐Repressing LINE1 Via Heterochromatin Erosion at Parturition.Advanced Science.DOI 10.1002/advs.202414682
29.Shoji Kawauchi; Sayo Horibe; Toru Tanaka; Naoto Sasaki; Junichi Kunimasa; et al.(2025). Disruption of small intestinal mucosal homeostasis in mice with amiodarone induced steatohepatitis.Scientific Reports.DOI 10.1038/s41598-025-04680-3
30.Xiangshuo Kong; Conghui Li; Jason Karpac.(2025).NF-κB restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila.bioRxiv(Cold Spring Harbor Laboratory).DOI 10.1101/2025.10.24.684223
31.Yuhang Zhou; Xingbo Wang; Jun Lyu; Qingfeng Ruan; Xiaoxuan Zeng; et al.(2025).Baitong Decoction Ameliorates DSS-Induced Colitis via Modulation of STING and JAK/STAT Pathways.ACS omega.DOI 10.1021/acsomega.5c06233
32.Dongmei Li; Yan Wei; Rui Yang; Xuan Luo; Yanzhu Liu; et al.(2025).An unrecognized mechanism of self-protection in degenerating neurons mediated by astrocytic YAP through Wnts/β-catenin/EAAT2 signaling in C9orf72-poly-GA mice.Theranostics. DOI 10.7150/thno.113599
力后，BNP就开始由心肌细胞合成，但是并不是直接合成BNP，而是先合成pro-BNP（可以翻译成前脑钠肽或者脑钠肽原），pro-BNP具有108个氨基酸，在血液中会分解，产生BNP，分解下来的片段是其N端的76个氨基酸，这76个氨基酸就是NT-proBNP ，也就是经常检测的那个指标，剩下的32个氨基酸才叫BNP。没有NT-BNP的概念，所谓的NT-BNP应该是指NT-proBNP。有点类似于C肽与胰岛素的关系。从这个角度上来讲，都叫脑钠肽不妥，脑钠肽英文应该叫rain（脑） natriuretic（利钠
电话:86-755-83501888-832 传真:86-755-89698555 手机:15889577842 13662968885(东莞) QQ:2850148531 联系人：方晓明 电话:0755-83501888-832 中国网址:http://www.esugimoto.com 日本威威VESSEL气动剪钳GT-NB30LW-10TM，GT-NB30LW-10SM，GT-NF05，GT-NF10，GT-NF15，GT-NK10
人神经营养因子3 (NT -3 ) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 神经营养因子3 (NT -3 ) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 人 脑源性神经营养因子（BDNF ） 水平。用纯化的 抗 - 脑源性神经营养因子（BDNF ） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次
