Thioredoxin/TXN/Trx重组大肠杆菌硫氧还蛋白
Recombinant Human Thioredoxin/TXN/Trx
货号CE85
TXN (E.coli,N-6His)
产品说明(Description)
Recombinant Human Thioredoxin is produced by our E.coli expression system and the target gene encoding Met1-Val105 is expressed with a 6His tag at the N-terminus.
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 1mM EDTA, 2mM DTT, pH7.2.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity : Measured by its ability to catalyze the reduction of insulin.
The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is 0.2-1 Abs/min/mg, as measured under the described conditions.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.