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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
生物素标记CCL15
- 目录编号:
500-P123GBT
- 抗体英文名:
Biotinylated Anti-Human MIP-5 (CCL15)
- 抗体名:
生物素标记CCL15
- 标记物:
生物素标记CCL15
- 适应物种:
人
- 应用范围:
WB ELISA
- 浓度:
Biotinylated Anti-Human MIP-5 (CCL15)
- 供应商:
上海研卉生物科技有限公司
- 保质期:
12个月
- 抗原来源:
10.1 kDa Recombinant Human MIP-5 (CCL15) (PeproTech catalog# 300-43)
- 库存:
500-P123GBT
- 宿主:
山羊
- 是否单克隆:
多克隆
- 规格:
25ug
产品编号: 500-P123GBT
资料来源:多克隆山羊
制备:用高纯度(>98%)重组hMIP-5预免疫的山羊血清制备。通过亲和层析纯化抗人MIP-5特异性抗体,然后进行生物素化。
免疫原:大肠杆菌衍生的10.1 kDa重组人MIP-5(CCL15)(PeproTech目录#300-43)
夹心ELISA:要通过夹心ELISA(使用100μl/孔抗体溶液)检测hMIP-5,需要浓度为0.25–1.0μg/ml的该抗体。该生物素化多克隆抗体与PeproTech的多克隆抗人MIP-5(500-P123G)结合作为捕获抗体,允许检测至少0.2–0.4 ng/孔的重组hMIP-5。
生物素化抗人MIP-5(CCL15)夹心ELISA
Western Blot:为了通过Western Blot分析检测hMIP-5,该抗体可在0.1-0.2µg/ml的浓度下使用。与兼容的二级试剂一起使用,重组hMIP-5在还原或非还原条件下的检测限为1.5-3.0 ng/lane。
| 500-P123G-50 | Goat Anti-Human MIP-5 (CCL15) | 50ug |
| 500-P123G-100 | Goat Anti-Human MIP-5 (CCL15) | 100ug |
| 500-P123G-1000 | Goat Anti-Human MIP-5 (CCL15) | 1000ug |
| 500-P123GBt-25 | Biotinylated Goat Anti-Human MIP-5 (CCL15) | 25ug |
| 500-P123GBt-50 | Biotinylated Goat Anti-Human MIP-5 (CCL15) | 50ug |
| 500-P123GBt-1000 | Biotinylated Goat Anti-Human MIP-5 (CCL15) | 1000ug |
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文献和实验Biotinylated Primer for Detecting Telomerase Activity Without Amplification
to be an attractive new target for designing anti-cancer agents, as well as an important diagnostic marker of human cancer (5 -7 ). For these reasons, it is necessary to develop telomerase assay methods that can be used for the quantitative measurement of telomerase
Western Blotting with Biotinylated Antibodies
ELECTROPHORESIS Guidelines for choosing the percent gel to be used for certain molecular weight proteins (based on 37:1 acralyamide: bis acraylamide ratio) 4-5% gels: > 250 kDa 7.5% gels: 250-120 kDa 10% gels: 120-40 kDa 13% gels: 40-15 kDa 15% gels: Gel
Detection of DNA Sequences Using Biotinylated Probes
It was found ( 1 ) that biotinylated analogs of dUTP, which contain a biotin molecule covalently bound to the C-5 position of the pyrimidine via an allylamine linker arm, can be used as substrates for DNA polymerase. Figure 1 shows the structure
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